A novel TaqMan®assay for Nosema ceranae quantification in honey bee, based on the protein coding gene Hsp70

Cilia, Giovanni ; Cabbri, Riccardo ; Maiorana, Giacomo ; Cardaio, Ilaria ; Dall'Olio, Raffaele ; Nanetti, Antonio (2018) A novel TaqMan®assay for Nosema ceranae quantification in honey bee, based on the protein coding gene Hsp70. European Journal of Protistology, 63 . pp. 44-50.
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Abstract

Nosema ceranae is now a widespread honey bee pathogen with high incidence in apiculture. Rapid and reliable detection and quantification methods are a matter of concern for research community, nowadays mainly relying on the use of biomolecular techniques such as PCR, RT-PCR or HRMA. The aim of this technical paper is to provide a new qPCR assay, based on the highly-conserved protein coding gene Hsp70, to detect and quantify the microsporidian Nosema ceranae affecting the western honey bee Apis mellifera. The validation steps to assess efficiency, sensitivity, specificity and robustness of the assay are described also.

Abstract
Document type
Article
Creators
CreatorsAffiliationORCID
Cilia, GiovanniConsiglio per la Ricerca in agricoltura e l’Analisi dell’economia agraria, Centro di Ricerca Agricoltura e Ambiente, Via di Saliceto 80, 40128 Bologna, Italy
Cabbri, RiccardoDepartment of Veterinary Medical Sciences, University of Bologna, Via Tolara di sopra 50, Ozzano del’Emilia, Bologna, Italy
Maiorana, GiacomoDepartment of Veterinary Medical Sciences, University of Bologna, Via Tolara di sopra 50, Ozzano del’Emilia, Bologna, Italy
Cardaio, IlariaConsiglio per la Ricerca in agricoltura e l’Analisi dell’economia agraria, Centro di Ricerca Agricoltura e Ambiente, Via di Saliceto 80, 40128 Bologna, Italy
Dall'Olio, RaffaeleConsiglio per la Ricerca in agricoltura e l’Analisi dell’economia agraria, Centro di Ricerca Agricoltura e Ambiente, Via di Saliceto 80, 40128 Bologna, Italy
Nanetti, AntonioConsiglio per la Ricerca in agricoltura e l’Analisi dell’economia agraria, Centro di Ricerca Agricoltura e Ambiente, Via di Saliceto 80, 40128 Bologna, Italy
Keywords
Apis melliferaHsp70 geneNosema ceranaeNosemosisqPCRTaqMan® assay
Subjects
DOI
https://doi.org/10.1016/j.ejop.2018.01.007
Deposit date
24 Aug 2018 09:48
Last modified
23 May 2019 09:44
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