README file

Data Set Title: OLEUM project. Revised method for determination of fatty acid ethyl esters in virgin olive oils. Data from in-house validation

Data Set Authors: Rosa Palagano (University of Bologna); Enrico Valli (University of Bologna); Matilde Tura (University of Bologna); Chiara Cevoli (University of Bologna);  Maria del Carmen Perez-Camino (Instituto de la Grasa, Spain); Wenceslao Moreda (Instituto de la Grasa, Spain); Alessandra Bendini (University of Bologna); Tullia Gallina Toschi (University of Bologna). 

Data Set Contact Person: Alessandra Bendini, http://orcid.org/0000-0002-6515-5519, alessandra.bendini@unibo.it.

Data Set License: this data set is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.

Publication Year: 2020

Project Info: OLEUM (Advanced solutions for assuring the overall authenticity and quality of olive oil), funded by European Union, Horizon 2020 Programme. Grant Agreement num. 635690; http://www.oleumproject.eu/.  

Data set contents
The data set consists of:
•	a numerical quantitative file saved both in .xlsx and .ods formats 
“OLEUM_Determination_of_Fatty_Acid_Ethyl_Esters.xlsx”
“OLEUM_Determination_of_Fatty_Acid_Ethyl_Esters.ods”
•	a README file 
“OLEUM_Determination_of_Fatty_Acid_Ethyl_Esters_README.rtf”

Data set Documentation
Abstract
This data set contains the underlying data of the scientific publication:
Palagano, R.; Valli, E.; Tura, M.; Cevoli, C.; Pérez-Camino, M.C.; Moreda, W.; Bendini, A.; Gallina Toschi, T. Fatty Acid Ethyl Esters in Virgin Olive Oils: In-House Validation of a Revised Method. Foods 2020, 9(7), 924; https://doi.org/10.3390/foods9070924. 
The content of fatty acid ethyl esters (FAEEs) is one of the quality parameters to define if an olive oil can be classified as extra virgin since these compounds are considered markers for virgin olive oils obtained from poor quality olives, but can be also indirect markers to detect soft deodorization treatment. The official protocol for determination (EU Reg. 61/2011) can be improved in terms of solvents amount and time of analysis.
An off-line HPLC-GC-FID method for FAEEs determination was developed, revising both the preparative step and the GC injector required by the official method. After optimization, the method was in-house validated by analyzing several parameters (linearity, LOD, LOQ, robustness, recovery, precision and accuracy). This data set contains the in-house validation results obtained.

Content of the file “OLEUM_Determination_of_Fatty_Acid_Ethyl_Esters.xlsx/.ods”: 
•	tab Linearity contains the data (values of two replicates for the ethyl esters and the mean value of 2 replicates for methyl heptadecanoate) obtained from the analysis of a solution of standards at different concentration, considering the range 2.5-50 ppm, and used to evaluate the linearity of the method for all the compounds considered.
•	tab Recovery contains the data (mean values of two replicates) measured to evaluate the recovery percentages for ethyl esters and methyl heptadecanoate (used as internal standard).
•	tab Robustness presents the areas measured injecting in HPLC a solution of standards (ethyl palmitate, ethyl linoleate, ethyl oleate, ethyl stearate) at two different mobile phase flows, namely 1 mL/min and 0.7 mL/min.
•	tab Inter-day precision presents the area values (mean of three replicates) measured analyzing a sample during three different days to evaluate the inter-day precision of the method.
File specifics
In the file “,” (comma) has been used as decimal separator.