SPARTEVs. Steroid hormones levels produced by cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes

Mateo-Otero, Yentel ; Yeste, Marc ; Roca, Jordi ; Llavanera, Marc ; Bucci, Diego ; Galeati, Giovanna ; Spinaci, Marcella ; Barranco, Isabel (2022) SPARTEVs. Steroid hormones levels produced by cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes. University of Bologna. DOI 10.6092/unibo/amsacta/7132. [Dataset]
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Abstract

Extracellular vesicles (EVs), defined as membrane-delimited nanoparticles (30-to-1000 nm), have emerged as essential cell-to-cell messengers, modulating several physio and pathological processes. Based on differences in size and biogenesis pathways, EVs released by functional cells are categorized into exosomes (small EVs) and microvesicles (large EVs). These EVs has been isolated from all reproductive fluids, including seminal plasma (SP), a complex fluid secreted mainly from accessory sexual glands that modulates sperm function and female reproductive tract immune environment. In pigs, SP has been found to modulate the ovary function, in terms of hormone secretion. This study investigated the effect of two seminal EVs subsets (small (S-EVs) and large (L-EVs) on cumulus cells during in vitro maturation (IVM) of porcine cumulus-cell-complexes COCs), in terms of steroid synthesis. This dataset contains the raw data of steroid hormones (progesterone and estradiol-17β) levels assessed in the IVM spent media when porcine COCs were IVM in presence of both seminal EVs-subsets at different concentrations (Low: 0.1 mg/mL; High: 0.2 mg/mL; as total protein concentration). The steroid hormones were assessed after 22 h and 44 h of IVM in the IVM spent media by radioimmunoassay.

Abstract
Tipologia del documento
Dataset
Autori
AutoreAffiliazioneORCID
Mateo-Otero, YentelUniversity of Girona, Spain0000-0002-5810-211X
Yeste, MarcUniversity of Girona, Spain0000-0002-2209-340X
Roca, JordiUniversity of Murcia, Spain0000-0002-4213-3093
Llavanera, MarcUniversity of Girona, Spain0000-0003-0685-7534
Bucci, DiegoUniversity of Bologna, Italy0000-0001-6276-2674
Galeati, GiovannaUniversity of Bologna, Italy0000-0001-5149-108X
Spinaci, MarcellaUniversity of Bologna, Italy0000-0002-8934-9035
Barranco, IsabelUniversity of Bologna, Italy0000-0001-9873-814X
Parole chiave
seminal plasama, extracellular vesicles, oocytes, hormones, cumulus cells
Settori scientifico-disciplinari
DOI
Contributors
Contributor
Affiliazione
ORCID
Tipo
Barranco, Isabel
University of Bologna, Italy
Contact person
Bucci, Diego
University of Bologna, Italy
Contact person
Data di deposito
09 Gen 2023 15:02
Ultima modifica
09 Gen 2023 15:02
Risorse collegate
Tipologia
Relazione
Identificativo
Nome del Progetto
SPARTEVs - Impact of extracellular vesicles isolated from seminal plasma upon Assisted Reproductive Technology (oocyte maturation, fertilization and embryo culture) in a mammalian model
Programma di finanziamento
EC - H2020
URI

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