SPARTEVs. Steroid hormones levels produced by cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes

Mateo-Otero, Yentel ; Yeste, Marc ; Roca, Jordi ; Llavanera, Marc ; Bucci, Diego ; Galeati, Giovanna ; Spinaci, Marcella ; Barranco, Isabel (2022) SPARTEVs. Steroid hormones levels produced by cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes. University of Bologna. DOI 10.6092/unibo/amsacta/7132. [Dataset]
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Extracellular vesicles (EVs), defined as membrane-delimited nanoparticles (30-to-1000 nm), have emerged as essential cell-to-cell messengers, modulating several physio and pathological processes. Based on differences in size and biogenesis pathways, EVs released by functional cells are categorized into exosomes (small EVs) and microvesicles (large EVs). These EVs has been isolated from all reproductive fluids, including seminal plasma (SP), a complex fluid secreted mainly from accessory sexual glands that modulates sperm function and female reproductive tract immune environment. In pigs, SP has been found to modulate the ovary function, in terms of hormone secretion. This study investigated the effect of two seminal EVs subsets (small (S-EVs) and large (L-EVs) on cumulus cells during in vitro maturation (IVM) of porcine cumulus-cell-complexes COCs), in terms of steroid synthesis. This dataset contains the raw data of steroid hormones (progesterone and estradiol-17β) levels assessed in the IVM spent media when porcine COCs were IVM in presence of both seminal EVs-subsets at different concentrations (Low: 0.1 mg/mL; High: 0.2 mg/mL; as total protein concentration). The steroid hormones were assessed after 22 h and 44 h of IVM in the IVM spent media by radioimmunoassay.

Document type
Mateo-Otero, YentelUniversity of Girona, Spain0000-0002-5810-211X
Yeste, MarcUniversity of Girona, Spain0000-0002-2209-340X
Roca, JordiUniversity of Murcia, Spain0000-0002-4213-3093
Llavanera, MarcUniversity of Girona, Spain0000-0003-0685-7534
Bucci, DiegoUniversity of Bologna, Italy0000-0001-6276-2674
Galeati, GiovannaUniversity of Bologna, Italy0000-0001-5149-108X
Spinaci, MarcellaUniversity of Bologna, Italy0000-0002-8934-9035
Barranco, IsabelUniversity of Bologna, Italy0000-0001-9873-814X
seminal plasama, extracellular vesicles, oocytes, hormones, cumulus cells
Barranco, IsabelUniversity of Bologna, Italy0000-0001-9873-814XContact person
Bucci, DiegoUniversity of Bologna, Italy0000-0001-6276-2674Contact person
Deposit date
09 Jan 2023 15:02
Last modified
09 Jan 2023 15:02
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DOIthis upload is supplement to
Project name
SPARTEVs - Impact of extracellular vesicles isolated from seminal plasma upon Assisted Reproductive Technology (oocyte maturation, fertilization and embryo culture) in a mammalian model
Funding program
EC - H2020

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