Author: Irene Frascari
Company: Università di Bologna

README file
Data Set Title: “NewTechAqua. Task 1.2. Comparison between 5 experimental diets containing various percentage of by-products from fishery and aquaculture on growth and fish health of European sea bass”
Data Set Author: Arianna Marchi (University of Bologna);
Data Set Contributors: Luca Parma (University of Bologna), Alessio Bonaldo (University of Bologna);
Data Set Contact Person: Arianna Marchi (University of Bologna), arianna.marchi5@unibo.it  
Data Set License: this data set is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/   
Publication Year: 2023
Project Info: NewTechAqua (New Technologies, Tools and Strategies for a Sustainable, Resilient and Innovative European Aquaculture), funded by European Union, Horizon 2020 Programme. Grant Agreement num. 862658; https://www.newtechaqua.eu.  
Data set Contents
The data set consists of:
•	2 tabular quantitative file saved in .xlsx format 
“NTA_TASK 1.2_Raw Data.xlsx”
“NTA_TASK 1.2_Plasma values.xlsx”
•	1 README file saved in .rtf format
“NTA_TASK 1.2_Raw Data-Plasma values_README.rtf”	
Data set Documentation
Abstract
The dataset contains raw data about new experimental European sea bass diets, obtained in the framework of Horizon 2020 NewTechAqua project. Five experimental diets were formulated to totally replace wild-caught fishmeal (FM), fish oil (FO) and soy protein (SP) using fisheries and aquaculture by-products, and alternative vegetable protein. The data were obtained before, after and during the laboratory trial, in order to test these experimental diets on European sea bass. The raw data include biological parameters, such as initial and final body weight, feed administration, Ambiental parameter, and values of plasma biochemistry, consisting in 25 blood parameters, generally used to detect animal health. All data were then processed with formulas and indices to obtain growth results and evaluate nutritional indices. 
Content of the files
•	file NTA_TASK 1.2_Raw Data.xlsx contains data of biological parameters considered during trial as initial and final body weight, feed administration, Ambiental parameter. All data were elaborated calculating all growth parameters, nutritional factor, somatic factors and digestibility and collected in a standard table.
o	Tab “Initial Weight” contains data about initial body weight, in grams, of 50 fish per 15 tanks.
o	Tab “Final Weight” contains data about final body weight of 50 per 15 tanks after 119 days. Data are expressed in grams.
o	Tab “Overall Performance_ Day 0-119” contains data about calculation and tables of all parameters and indices collected during the trial.
o	Tab “Daily Feed Intake” contains data about grams of feed administrated to animals and the quantity of lefts. 
o	Tab “Feeding consumption” contains data about data of on the theoretical amount of feed assumed to be ingested during trial by single animal. 
o	Tab “Theoritical Daily Growth” contains data about calculation of the theoretical daily growth of each European sea bass.
o	Tab “Dead” contains data about mortality, counting the number of death animal during the trial.
o	Tab “Somatic index” contains data about somatic parameters, as length, and weight of liver and fat. Expressed in grams.
o	Tab “Length” contains data about measurement in centimeter of each animal. 
•	file NTA_TASK 1.2_Plasma values.xlsx contains raw and evaluated values of plasma biochemistry, consisting in 25 blood parameters, generally used to detect animal health. More specifically, the levels of: glucose (GLUC or GLU, mg/dl), urea (mg/dl), creatine (CREA mg/dl), uric acid (URICO mg/dl), total bilirubin (BILT mg/dl), cholesterol (COL mg/dl), high density lipoprotein (HDL, mg/dl), triglycerides (TRIG, mg/dl), total protein (TP, g/dl), albumin (ALB g/dl), aspartate aminotransferase (AST, U/L), alanine transaminase (ALT, U/L), alkaline phosphatase (ALP, U/L), creatine kinase (CK, IU/L), lactate dehydrogenase (LDH, IU/L), calcium (CA, mg/dl), phosphorus (P, mg/dl), potassium (K, mEq/L) sodium (Na, mEq/L), iron (Fe, mcg/dl), chloride (Cl, mEq/L), magnesium (Mg, mg/dl) were determined in the plasma. The Albumine/Globulin (A/G or AG) ratio, lactate (LATTA or LAT, mg/dl) and Current Calcium (Ca.COR or CA CORR, mg/dl) were calculated.
Notes 
The data were presented in the following publication: 
Marchi, A.; Bonaldo, A.; Di Biase, A.; Cerri, R.; Scicchitano, D.; Nanetti, E.; Candela, M.; Picone, G.; Capozzi, F.; Dondi, F.; Gatta, P. P.; Parma, L. Towards a Free Wild-Caught Fishmeal, Fish Oil and Soy Protein in European Sea Bass Diet Using by-Products from Fishery and Aquaculture. Aquaculture 2023, 573, 739571. https://doi.org/10.1016/j.aquaculture.2023.739571.
Methodologies 
Five experimental diets were formulated to totally replace wild-caught fishmeal (FM), fish oil (FO) and soy protein (SP) using fisheries and aquaculture by-products, and alternative vegetable protein. Wild-caught FM and krill meal (FM prime, krill meal) were totally replaced by adding a combination of FM trimming and shrimp meal (0%FM,100%FO, 0FM100FO). Wild-caught FO (FO Extra) was totally replaced with FO trimming (0 %FM, 0%FO, 0FM0FO). SP (soybean meal and soy protein concentrate) were half (0%FM, 0%FO, 50%SP, 0FM0FO50SP) and totally replaced (0%FM, 0%FO, 0%SP, 0FM0FO0SP) with a blend of corn gluten and wheat gluten. Diets were extruded at industrial level with a diameter of 3 mm. Feeds were produced by AIA – Agricola Italiana Alimentare S.p.A. (Verona, Italy). 
List of ingredients of the experimental diets:
Ingredients, % of the diet | C | 0FM100FO | 0FM0FO | 0FM0FO50SP | 0FM0FO0SP
Fish meal Prime^1 | 25.0 | – | – | – | –
Fish meal Trimming^2 | – | 25.0 | 25.0 | 25.0 | 25.0
Krill meal^3 | 2.50 | – | – | – | –
Shrimps meal^4 | – | 2.50 | 2.50 | 8.50 | 8.50
Fish oil extra^5 | 14.6 | 14.25 | – | – | –
Fish oil trimming^6 | – | – | 14.25 | 12.5 | 12.5
Wheat meal | 14.5 | 14.3 | 14.4 | 14.0 | 18.2
Soybean meal^7 | 19.2 | 19.2 | 19.2 | 9.6 | –
Soy protein concentrate^8 | 8.00 | 8.00 | 8.00 | 4.00 | –
Corn gluten^9 | 4.00 | 4.00 | 4.00 | 10.0 | 15.5
Wheat gluten^10 | – | 2.60 | 2.50 | 2.75 | 9.50
Sunflower seed meal feed, dehulled | 5.00 | 4.00 | 4.00 | 5.00 | 5.00
Guar germ meal | 6.00 | 5.00 | 5.00 | 5.00 | –
Yeasts^11 | – | – | – | – | 2.00
Microalgae^11 | | | | 2.50 | 2.50
Vitamins/mineral premix^13 | 1.20 | 1.20 | 1.20 | 1.20 | 1.20
^1Origin: Chile; Composition: protein 67%, lipid 10%, ash 14%. Antioxidant: butylated hydroxyanisole, BHA, 40 ppm; Butylated hydroxytoluene BHT, 200 ppm.
^2Obtained from Atlantic mackerel (Scomber scombrus) and sardine (Sardina pilchardus). Origin: Morocco; Composition: protein 63%, lipid 10%, ash 20%. BHA, 80 ppm; BHT, 80 ppm.
^3Composition: protein 54%, lipid, 18%, ash 13%.
^4Obtained from whiteleg shrimp (Litopenaeus vannamei) heads; Composition: protein 52%, lipid 8%, ash 20%.
^5Origin: Chile. Antioxidant: BHA 90 ppm, BHT 80 ppm, propyl gallate, PG 40 ppm.
^6Obtained from Atlantic salmon (Salmo salar). Origin: EU. Antioxidant: BHA 70 ppm, BHT 145 ppm, PG 40 ppm.
^7Origin: Italy (gmo-free). Composition: protein 49%, lipid, 1%.
^8Origin: Serbia. Composition: protein, 61%, lipid, 0.3%.
^9Origin: Italy.
^10Origin: Italy.
^11Saccaromices cerevisiae.
^12Obtained from processed Schizochytrium (Corbion, Brazil).
^13Vitamins/mineral premix (IU or mg kg−1 diet): vitamin A (12000 IU); vitamin D (2000 IU); vitamin E (160 mg); vitamin C (160 mg); Manganese (40 mg); Zinc (55 mg); Iron (20 mg); Copper (8 mg); Iodine (2 mg); selenium (0.15 mg); BHA (150 mg); PG (75 mg).
For more information about the five experimental diets consult: Marchi, A.; Bonaldo, A.; Di Biase, A.; Cerri, R.; Scicchitano, D.; Nanetti, E.; Candela, M.; Picone, G.; Capozzi, F.; Dondi, F.; Gatta, P. P.; Parma, L. Towards a Free Wild-Caught Fishmeal, Fish Oil and Soy Protein in European Sea Bass Diet Using by-Products from Fishery and Aquaculture. Aquaculture 2023, 573, 739571. https://doi.org/10.1016/j.aquaculture.2023.739571.