- Script: 

setImageType('FLUORESCENCE');
selectAnnotations();
runPlugin('qupath.imagej.detect.cells.WatershedCellDetection', '{"detectionImage": "20X DAPI",  "requestedPixelSizeMicrons": 0.5,  "backgroundRadiusMicrons": 8.0,  "medianRadiusMicrons": 0.0,  "sigmaMicrons": 1.5,  "minAreaMicrons": 10.0,  "maxAreaMicrons": 400.0,  "threshold": 250.0,  "watershedPostProcess": true,  "cellExpansionMicrons": 2.0,  "includeNuclei": true,  "smoothBoundaries": true,  "makeMeasurements": true}');
runObjectClassifier("ECAD+","Ki67+");


-----------------------------------------------------------------------------------------

- Object classifier for ECAD:

{
  "object_classifier_type": "SimpleClassifier",
  "function": {
    "classifier_fun": "ClassifyByMeasurementFunction",
    "measurement": "Cytoplasm: Cy5 mean",
    "pathClassBelow": {
      "name": "ECAD-",
      "colorRGB": -65536
    },
    "pathClassEquals": {
      "name": "ECAD+",
      "colorRGB": -1
    },
    "pathClassAbove": {
      "name": "ECAD+",
      "colorRGB": -1
    },
    "threshold": 80.53976002641907
  },
  "pathClasses": [
    {
      "name": "ECAD-",
      "colorRGB": -65536
    },
    {
      "name": "ECAD+",
      "colorRGB": -1
    }
  ],
  "filter": "DETECTIONS_ALL",
  "timestamp": 1704450694517
}



-----------------------------------------------------------------------------------------


- Object classifier for Ki67: 
{
  "object_classifier_type": "SimpleClassifier",
  "function": {
    "classifier_fun": "ClassifyByMeasurementFunction",
    "measurement": "Nucleus: FITC mean",
    "pathClassBelow": {
      "name": "Ki67-",
      "colorRGB": -16711936
    },
    "pathClassEquals": {
      "name": "Ki67+",
      "colorRGB": -65281
    },
    "pathClassAbove": {
      "name": "Ki67+",
      "colorRGB": -65281
    },
    "threshold": 845.0
  },
  "pathClasses": [
    {
      "name": "Ki67-",
      "colorRGB": -16711936
    },
    {
      "name": "Ki67+",
      "colorRGB": -65281
    }
  ],
  "filter": "DETECTIONS_ALL",
  "timestamp": 1709803847566
}