Dataset Title: “ECLectic. WP3 Task 3.1. Versatile Electrochemiluminescence Imaging Platform for Multiplexed Biosensing and Point-of-Care Application”. 
Dataset Author: Stephania Rodríguez Muiña (BCMaterials and University of the Basque Country-UPV/EHU), ORCID (0009-0006-3196-8964); Gabriele Giagu (University of Bologna), ORCID (0009-0003-9755-2986); Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072); Javier del Campo García (BCMaterials and University of the Basque Country-UPV/EHU), ORCID (0000-0002-3637-5782). 
Dataset Contributors: Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072).              
Dataset Contact Person: Javier del Campo García (BCMaterials and University of the Basque Country-UPV/EHU), ORCID (0000-0002-3637-5782), javier.delcampo@bcmaterials.net; Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072), g.valenti@unibo.it.
Dataset License: this dataset is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.
Publication Year: 2026
Project Info: ECLectic, funded by European Union, MSCA Doctoral Network Horizon Europe programme. Grant Agreement num. 101119951
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Folder "Calibration_single_SAP_(B)" contents

The folder consists of: 
• 90 captured image frames
"B1_0nM_frameroi.png" – "B3_0nM_frameroi.png"
"B1_2V_0nM_png.png" – "B3_2V_0nM_png.png"
"B1_2V_0nM_png_processed.png" – "B3_2V_0nM_png_processed.png"
"B1_2V_0nM_tiff.tiff" – "B3_2V_0nM_tiff.tiff"
"B1_bg_0nM_png.png" – "B3_bg_0nM_png.png"
"B1_bg_0nM_tiff.tiff" – "B3_bg_0nM_tiff.tiff"
"B4_0_033nM_frameroi.png" – "B6_0_033nM_frameroi.png"
"B4_2V_0_033nM_png.png" – "B6_2V_0_033nM_png.png"
"B4_2V_0_033nM_png_processed.png" – "B6_2V_0_033nM_png_processed.png"
"B4_2V_0_033nM_tiff.tiff" – "B6_2V_0_033nM_tiff.tiff"
"B4_bg_0_033nM_png.png" – "B6_bg_0_033nM_png.png"
"B4_bg_0_033nM_tiff.tiff" – "B6_bg_0_033nM_tiff.tiff"
"B7_0_1nM_frameroi.png" – "B9_0_1nM_frameroi.png"
"B7_2V_0_1nM_png.png" – "B9_2V_0_1nM_png.png"
"B7_2V_0_1nM_png_processed.png" – "B9_2V_0_1nM_png_processed.png"
"B7_2V_0_1nM_tiff.tiff" – "B9_2V_0_1nM_tiff.tiff"
"B7_bg_0_1nM_png.png" – "B9_bg_0_1nM_png.png"
"B7_bg_0_1nM_tiff.tiff" – "B9_bg_0_1nM_tiff.tiff"
"B10_0_33nM_frameroi.png" – "B12_0_33nM_frameroi.png"
"B10_2V_0_33nM_png.png" – "B12_2V_0_33nM_png.png"
"B10_2V_0_33nM_png_processed.png" – "B12_2V_0_33nM_png_processed.png"
"B10_2V_0_33nM_tiff.tiff" – "B12_2V_0_33nM_tiff.tiff"
"B10_bg_0_33nM_png.png" – "B12_bg_0_33nM_png.png"
"B10_bg_0_33nM_tiff.tiff" – "B12_bg_0_33nM_tiff.tiff"
"B13_1nM_frameroi.png" – "B15_1nM_frameroi.png"
"B13_2V_1nM_png.png" – "B15_2V_1nM_png.png"
"B13_2V_1nM_png_processed.png" – "B15_2V_1nM_png_processed.png"
"B13_2V_1nM_tiff.tiff" – "B15_2V_1nM_tiff.tiff"
"B13_bg_1nM_png.png" – "B15_bg_1nM_png.png"
"B13_bg_1nM_tiff.tiff" – "B15_bg_1nM_tiff.tiff"
• 2 Excel files
"Calibration_single_SAP.csv"
"OCP-2s_CA_2V-5s_electrochemical_data_single_SAP.csv"
• 1 Readme file
"Calibration_single_SAP_(B)_README.txt"

Abstract
This dataset contains electrochemical data obtained from ECL chronoamperometry experiments using magnetic bead–antibody–[Ru(bpy)₃]²⁺ conjugates at varying concentrations, spotting a single concentration in circular-shaped screen-printed electrodes. The dataset aims to quantify the luminescent response of the system and support the image-based quantitative analytical methods of the platform.

The experimental conditions of the chronoamperometry experiments were:
	- Analyte: Magnetic bead-Ab-[Ru(bpy)₃]²⁺ at concentrations of 0, 0.033, 0.1, 0.33 or 1 nM
	- Coreactant/Electrolyte: 180 mM tripropylamine (TPrA) in 0.3 M phosphate buffer, pH 6.8
	- Electrode Configuration (Screen-printed electrodes):
		- Working electrode: GST4500 C
		- Reference electrode: EDAG 725A Ag
		- Counter electrode: GST4500 C
	- Chronoamperometric Protocol:
		- Frameroi and background images (bg): No electrochemical signal applied; images used for ROI selection and background reference.
 		- ECL recordings: 
			- Open circuit potential (OCP) for 2 seconds 
			- Chronoamperometry (CA) step to 2 V vs Ag for 5 seconds 
	- Temperature = room temperature
	- Recording parameters:
		Low-light OV9281 monochrome camera settings: analogue gain 15×, duration 8 s, framerate 1/8 frames/s, resolution 1280×800 pixels, brightness 0, contrast 1, noise reduction off, sensor mode 1280×800-Y10_1X10/RAW with YUV420 preview, buffer count = 10.
Note: For frameroi images, all settings except resolution were set to automatic; resolution fixed at 1280×800 pixels.
	- Processing done for visualisation purposes (only applied to processed frames) = automatic brightness and contrast adjustment.


Content of the files 
• B1_0nM_frameroi.png" – "B3_0nM_frameroi.png" contains PNG image frames of the initial electrode image used for ROI selection during image analysis for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0 nM concentration.
• "B1_2V_0nM_png.png" – "B3_2V_0nM_png.png" contains PNG image frames of the ECL signal recorded during OCP-CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0 nM concentration.
• "B1_2V_0nM_png_processed.png" – "B3_2V_0nM_png_processed.png" contains processed PNG image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0 nM concentration.
• "B1_2V_0nM_tiff.tiff" – "B3_2V_0nM_tiff.tiff" contains TIFF image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0 nM concentration.
• "B1_bg_0nM_png.png" – "B3_bg_0nM_png.png" contains PNG background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0 nM concentration.
• "B1_bg_0nM_tiff.tiff" – "B3_bg_0nM_tiff.tiff" contains TIFF background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0 nM concentration.
"B4_0_033nM_frameroi.png" – "B6_0_033nM_frameroi.png" contains PNG image frames of the initial electrode image used for region-of-interest (ROI) selection during image analysis for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.033 nM concentration.
• "B4_2V_0_033nM_png.png" – "B6_2V_0_033nM_png.png" contains PNG image frames of the electrochemiluminescence (ECL) signal recorded during open-circuit potential–chronoamperometry (OCP–CA) experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.033 nM concentration.
• "B4_2V_0_033nM_png_processed.png" – "B6_2V_0_033nM_png_processed.png" contains processed PNG image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.033 nM concentration.
• "B4_2V_0_033nM_tiff.tiff" – "B6_2V_0_033nM_tiff.tiff" contains TIFF image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.033 nM concentration.
• "B4_bg_0_033nM_png.png" – "B6_bg_0_033nM_png.png" contains PNG background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.033 nM concentration.
• "B4_bg_0_033nM_tiff.tiff" – "B6_bg_0_033nM_tiff.tiff" contains TIFF background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.033 nM concentration.
• "B7_0_1nM_frameroi.png" – "B9_0_1nM_frameroi.png" contains PNG image frames of the initial electrode image used for region-of-interest (ROI) selection during image analysis for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.1 nM concentration.
• "B7_2V_0_1nM_png.png" – "B9_2V_0_1nM_png.png" contains PNG image frames of the electrochemiluminescence (ECL) signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.1 nM concentration.
• "B7_2V_0_1nM_png_processed.png" – "B9_2V_0_1nM_png_processed.png" contains processed PNG image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.1 nM concentration.
• "B7_2V_0_1nM_tiff.tiff" – "B9_2V_0_1nM_tiff.tiff" contains TIFF image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.1 nM concentration.
• "B7_bg_0_1nM_png.png" – "B9_bg_0_1nM_png.png" contains PNG background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.1 nM concentration.
• "B7_bg_0_1nM_tiff.tiff" – "B9_bg_0_1nM_tiff.tiff" contains TIFF background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.1 nM concentration.
• "B10_0_33nM_frameroi.png" – "B12_0_33nM_frameroi.png" contains PNG image frames of the initial electrode image used for region-of-interest (ROI) selection during image analysis for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.33 nM concentration.
• "B10_2V_0_33nM_png.png" – "B12_2V_0_33nM_png.png" contains PNG image frames of the electrochemiluminescence (ECL) signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.33 nM concentration.
• "B10_2V_0_33nM_png_processed.png" – "B12_2V_0_33nM_png_processed.png" contains processed PNG image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.33 nM concentration.
• "B10_2V_0_33nM_tiff.tiff" – "B12_2V_0_33nM_tiff.tiff" contains TIFF image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.33 nM concentration.
• "B10_bg_0_33nM_png.png" – "B12_bg_0_33nM_png.png" contains PNG background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.33 nM concentration.
• "B10_bg_0_33nM_tiff.tiff" – "B12_bg_0_33nM_tiff.tiff" contains TIFF background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 0.33 nM concentration.
• "B13_1nM_frameroi.png" – "B15_1nM_frameroi.png" contains PNG image frames of the initial electrode image used for region-of-interest (ROI) selection during image analysis for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 1 nM concentration.
• "B13_2V_1nM_png.png" – "B15_2V_1nM_png.png" contains PNG image frames of the electrochemiluminescence (ECL) signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 1 nM concentration.
• "B13_2V_1nM_png_processed.png" – "B15_2V_1nM_png_processed.png" contains processed PNG image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 1 nM concentration.
• "B13_2V_1nM_tiff.tiff" – "B15_2V_1nM_tiff.tiff" contains TIFF image frames of the ECL signal recorded during OCP–CA experiments for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 1 nM concentration.
• "B13_bg_1nM_png.png" – "B15_bg_1nM_png.png" contains PNG background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 1 nM concentration.
• "B13_bg_1nM_tiff.tiff" – "B15_bg_1nM_tiff.tiff" contains TIFF background images acquired for magnetic bead–Ab–[Ru(bpy)₃]²⁺ at 1 nM concentration.
• "Calibration_single_SAP.csv" contains ECL intensity data extracted from the selected ROI of the image frames.
• "OCP-2s_CA_2V-5s_electrochemical_data_single_SAP.csv" contains electrochemical data of the OCP-CA experiments.

File Specifics
• 90 captured image frames
The dataset includes image files in PNG and TIFF formats captured and saved using custom-developed platform software.	
• 2 Excel files
The dataset includes two data files provided in CSV (Comma-Separated Values) format, containing the numerical outputs generated by the platform. The files were combined using Microsoft Excel for Microsoft 365 (MSO Version 2111, Build 16.0.19426.20260) and exported as CSV files.
	• Field delimiter: « , » (comma)
	• File format: Plain text CSV 

Filename Convention
• 90 captured image frames
Each filename follows the patterns:
[SampleID]_[Concentration]_frameroi.[FileExtension]; [SampleID]_[Voltage]_[Concentration]_[FileType].[FileExtension]; [SampleID]_[Voltage]_[Concentration]_[FileType]_processed.[FileExtension]; [SampleID]_bg_[Concentration]_[FileType].[FileExtension]; 

Where:
	- SampleID: Sequential identifier of the measurement type B (single magnetic bead-Ab-[Ru(bpy)₃]²⁺ experiments).
	- frameroi: Indicates a frame used for ROI selection during analysis.
	- Concentration: Nominal concentration of magnetic bead-Ab-[Ru(bpy)₃]²⁺.
	- Voltage: Voltage applied during the chronoamperometric measurement (only when applied).
	- processed: Indicates that the frame has been processed by the software.
	- FileType/FileExtension: PNG or TIFF.
	- bg: Indicates a background image.

Codebook of Variables
• 2 Excel files
The dataset includes two tabular quantitative files in CSV (.csv) format. The columns in each file represent the following variables:

1. "OCP-2s_CA_2V-5s_electrochemical_data_single_SAP.csv":
• Time (s) = Time elapsed from the start of the electrochemical measurement, in seconds (s).
• Measured Potential (V) = Potential response measured during open-circuit potential (OCP), in volts (V), for each experiment.
• Measured Current (A) = Electrical current recorded in response to the applied potential during chronoamperometry (CA), in ampere (A), for each experiment.

2. "Calibration_single_SAP.csv":
• Experiment = Identifier of the experiment number.	
• [Ab-Ru] (nM) = Nominal concentration of magnetic bead-Ab-[Ru(bpy)₃]²⁺, in nanomolar (nM) 	
• ECL_png_raw (counts) = Raw ECL intensity measured from the selected ROI in PNG image frames, in counts.
• ECL_png_raw-bg (counts) = Background-subtracted ECL intensity measured from the selected ROI in PNG image frames, in counts.	
• ECL_tiff_raw (counts) = Raw ECL intensity measured from the selected ROI in TIFF image frames, in counts.
• ECL_tiff_raw-bg (counts) = Background-subtracted ECL intensity measured from the selected ROI in TIFF image frames, in counts.

