Dataset Title: “ECLectic. WP3 Task 3.1. Versatile Electrochemiluminescence Imaging Platform for Multiplexed Biosensing and Point-of-Care Application”. 
Dataset Author: Stephania Rodríguez Muiña (BCMaterials and University of the Basque Country-UPV/EHU), ORCID (0009-0006-3196-8964); Gabriele Giagu (University of Bologna), ORCID (0009-0003-9755-2986); Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072); Javier del Campo García (BCMaterials and University of the Basque Country-UPV/EHU), ORCID (0000-0002-3637-5782). 
Dataset Contributors: Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072).              
Dataset Contact Person: Javier del Campo García (BCMaterials and University of the Basque Country-UPV/EHU), ORCID (0000-0002-3637-5782), javier.delcampo@bcmaterials.net; Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072), g.valenti@unibo.it.
Dataset License: this dataset is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.
Publication Year: 2026
Project Info: ECLectic, funded by European Union, MSCA Doctoral Network Horizon Europe programme. Grant Agreement num. 101119951
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Folder "C_CV_0-2V_100mV_s_multiplex_SAP" contents

The folder consists of: 
• 42 captured image frames
"0_frameroi.png"
"1_bg_frame.png"
"frame_0000.png" - "frame_0039.png"
• 1 Excel file
"C_CV_0-2V_100mV_s_multiplex_SAP.csv"
• 1 video file
"video.mp4"
• 1 Readme file
"C_CV_0-2V_100mV_s_multiplex_SAP_README.txt"

Abstract
This dataset contains electrochemical data acquired from an ECL cyclic voltammetry experiment using magnetic bead–antibody–[Ru(bpy)₃]²⁺ conjugates at varying concentrations, spotting five different concentrations (0, 0.033, 0.1, 0.33 and 1 nM) in a rectangular-shaped screen-printed electrode. The dataset is intended to validate the coordinated operation and low-light detection capabilities of the platform.

The experimental conditions of the cyclic voltammetry experiment was: 
	- Analyte: Magnetic bead-Ab-[Ru(bpy)₃]²⁺ at concentrations of 0, 0.033, 0.1, 0.33 or 1 nM
	- Coreactant/Electrolyte: 180 mM tripropylamine (TPrA) in 0.3 M phosphate buffer, pH 6.8
	- Electrode Configuration (Screen-printed electrode):
		- Working electrode: GST4500 C
		- Reference electrode: EDAG 725A Ag
		- Counter electrode: GST4500 C
	- Chronoamperometric Protocol:
		- Frameroi and background images (bg): No electrochemical signal applied; images used for ROI selection and background reference.
 		- ECL recordings: 
			- Cyclic voltammetry (CV) from 0 V to 2 V at 100 mV/s
	- Temperature = room temperature
	- Recording parameters:
		OV9281 monochrome camera settings: analogue gain 15×, duration 40 s, framerate 1 frames/s, resolution 1280×800 pixels, brightness 0, contrast 1, noise reduction off, sensor mode 1280×800-Y10_1X10/RAW with YUV420 preview, buffer count = 10.
Note: For frameroi images, all settings except resolution were set to automatic; resolution fixed at 1280×800 pixels.

Content of the files
• "0_frameroi.png" contains a PNG image frame of the initial electrode image used for ROI selection during image analysis.
• "1_bg_frame.png" contains a PNG background image acquired.
• "frame_0000.png" - "frame_0039.png" contains PNG image frames of the ECL signal recorded during the CV experiment.
• "video.mp4" video file generated from frame data.
• "B_CV_0-2V_100mV_s_single_SAP.csv" contains ECL data of the CV experiments.

File Specifics
• 42 captured image frames and 1 video file
The dataset includes image files in PNG, TIFF and MP4 formats captured and saved using custom-developed platform software.	
• 1 Excel file
The dataset includes two data files provided in CSV (Comma-Separated Values) format, containing the numerical outputs generated by the platform. The files were combined using Microsoft Excel for Microsoft 365 (MSO Version 2111, Build 16.0.19426.20260) and exported as CSV files.
	• Field delimiter: « , » (comma)
	• File format: Plain text CSV 

Codebook of Variables
• 1 Excel file 
"C_CV_0-2V_100mV_s_multiplex_SAP.csv"
The dataset includes one tabular quantitative file in CSV (.csv) format with 15 columns:
• Applied Potential (V) = Potential applied during the CV experiment, in volts (V).
• Measured Current (A) = Electrical current recorded in response to the applied potential during CV, in ampere (A).
• Frame Index = Identifier of the frame number.	
• Frame Time (s) = Corresponding time of the frame, considering the framerate, in seconds (s).  
• Frame Potential (V) = Corresponding applied potential to the frame, in volts (V).	
• ECL_png_raw (counts) = Raw ECL intensity measured from the selected ROI in PNG image frames, in counts, per selected ROI.
• ECL_png_raw-bg (counts) = Background-subtracted ECL intensity measured from the selected ROI in PNG image frames, in counts, per selected ROI.

