Dataset Title: Nano-ImmunoEra. WP2 T2.4. ECL quantification of cTnI on SPCE_Cu3(HHTP)2.
Dataset Author: Alenzo Murray (University of Western Cape), ORCID (0000-0002-6151-1774);
               
Dataset Contributors: Priscilla Baker (University of Western Cape), ORCID (0000-0002-8878-2670);
		                   Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072).

Dataset Contact Person: Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072), g.valenti@unibo.it
Dataset License: this dataset is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.
Publication Year: 2026
Project Info: NanoimmunoEra Project, funded by European Union’s MSCA Staff exchange Horizon Europe programme, Horizon 2020 Programme. Grant Agreement Number 101086341; https://nanoimmunoera-project.eu.

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Folder "Different MOF loadings" Contents
The folder consists of:
• 1 tabular quantitative file saved in .csv format
“Effect_of_Cu3-HHTP-2_loading_on_SPCE_ECL.csv”
• 1 README file
“Effect_of_Cu3-HHTP-2_loading_on_SPCE_ECL_README.txt”

Folder Documentation

Abstract
This folder stores data related to the Task 2.4_Electrochemical and ECL performance of ink-jet printed electrodes within WorkPackage2_ Production_Characterization_Materials for Improved ECL-based Biosensing,  in the NanoimmunoEra Horizon 2020 project. To establish optimal Cu₃(HHTP)₂ deposition for ECL enhancement, aqueous dispersions spanning 1–5 mg/mL were sonicated for homogeneity before precise 15 μL drop-casting onto 4 mm-diameter screen-printed carbon electrodes (SPCEs, DropSens C110), followed by overnight room-temperature drying to ensure uniform thin-film formation.​

Electrochemiluminescence measurements in PBS (10 mM, pH 7.0) with 100 μM [Ru(bpy)₃]²⁺ revealed progressive signal intensification up to 2 mg/mL loading (20.8 a.u.), reflecting improved conductivity, expanded electroactive surface area, and enhanced luminophore preconcentration within the c-MOF's porous nanorod architecture (confirmed via SEM/AFM). Beyond this optimum, ECL intensity declined sharply (e.g., 6.2 a.u. at 5 mg/mL), attributable to excessive film thickness impeding charge transport, analyte diffusion limitations, and potential aggregation-induced insulation.

ECL measurements utilized a Stat ECL potentiostat (Metrohm, Switzerland) in 40 μL droplets of PBS (10 mM, pH 7.0) containing 100 μM [Ru(bpy)₃]²⁺, applying linear sweep voltammetry from 0 to 1.3 V vs. Ag/AgCl at a scan rate of 0.05 V/s (50 mV/s).


Content of the files:
• file "Effect_of_Cu3-HHTP-2_loading_on_SPCE_ECL.csv" contains raw ECL intensity data (peak of the ECL emission during the Linear Sweep Voltammetry) corresponding to five distinct c-MOF loading levels (1, 2, 3, 4, 5 mg/mL).
  
File specifics
After their collection, data have been exported as .txt files and elaborated through OriginPro 2015 software. The elaborated results have been copied into an Excel file and converted into a .csv file. In particular, to create the .csv files, “Microsoft Excel for Microsoft 365” version 2302 Build 16.0.16130.20332 (portable) was used, with the following specifics:
• character set UTF-8,
• field delimiter « ; » (semicolon),
• decimal separator « , » (comma)
• text delimiter « " » (quotes)
