Dataset Title: Nano-ImmunoEra. WP2 T2.4. ECL quantification of cTnI on SPCE_Cu3(HHTP)2.
Dataset Author: Alenzo Murray (University of Western Cape), ORCID (0000-0002-6151-1774);
               
Dataset Contributors: Priscilla Baker (University of Western Cape), ORCID (0000-0002-8878-2670);
		                   Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072).

Dataset Contact Person: Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072), g.valenti@unibo.it
Dataset License: this dataset is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.
Publication Year: 2026
Project Info: NanoimmunoEra Project, funded by European Union’s MSCA Staff exchange Horizon Europe programme, Horizon 2020 Programme. Grant Agreement Number 101086341; https://nanoimmunoera-project.eu.

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Folder "Effect of PBS pH" Contents
The folder consists of:
• 1 tabular quantitative file saved in .csv format
Effect_of_PBS_pH_on_SPCE_Cu3-HHTP-2.csv
• 1 README file
Effect_of_PBS_pH_on_SPCE_Cu3-HHTP-2_README.txt

Folder Documentation

Abstract
This folder stores data related to the Task 2.4_Electrochemical and ECL performance of ink-jet printed electrodes within WorkPackage2_ Production_Characterization_Materials for Improved ECL-based Biosensing,  in the NanoimmunoEra Horizon 2020 project. 
Effect of PBS pH on ECL was investigated using Cu₃(HHTP)₂-modified SPCEs (2 mg/mL loading) in 40 μL PBS (10 mM) with 100 μM [Ru(bpy)₃]²⁺ across pH 6.0–8.0 (intervals: 6.0, 6.5, 7.0, 7.5, 8.0).
Signal intensity escalated from 5.09 a.u. (pH 6) to a maximum of 24.32 a.u. at pH 8, driven by deprotonation of HHTP ligands, improves the Ru(bpy)₃²⁺ oxidation, optimizing the ECL kinetics. 
Despite pH 8 peak, pH 7.0 was selected for biosensor operation to preserve anti-cTnI antibody stability during the immunoassay.
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ECL measurements utilized a Stat ECL potentiostat (Metrohm, Switzerland) in 40 μL droplets of PBS (10 mM, pH 7.0) containing 100 μM [Ru(bpy)₃]²⁺, applying linear sweep voltammetry from 0 to 1.3 V vs. Ag/AgCl at a scan rate of 0.05 V/s (50 mV/s).


Content of the files:
• file "Effect_of_PBS_pH_on_SPCE_Cu3-HHTP-2.csv" lists peak ECL emission intensities (a.u.) recorded at the maximum of the emission profile during linear sweep voltammetry for five PBS pH conditions (6, 6.5, 7, 7.5, 8).

File specifics
After their collection, data have been exported as .txt files and elaborated through OriginPro 2015 software. The elaborated results have been copied into an Excel file and converted into a .csv file. In particular, to create the .csv files, “Microsoft Excel for Microsoft 365” version 2302 Build 16.0.16130.20332 (portable) was used, with the following specifics:
• character set UTF-8,
• field delimiter « ; » (semicolon),
• decimal separator « , » (comma)
• text delimiter « " » (quotes).