﻿Dataset Title: Nano-ImmunoEra. WP2 T2.4. ECL quantification of cTnI on SPCE_Cu3(HHTP)2.
Dataset Author: Alenzo Murray (University of Western Cape), ORCID (0000-0002-6151-1774);
               
Dataset Contributors: Priscilla Baker (University of Western Cape), ORCID (0000-0002-8878-2670);
		              Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072).

Dataset Contact Person: Giovanni Valenti (University of Bologna), ORCID (0000-0002-6223-2072), g.valenti@unibo.it
Dataset License: this dataset is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.
Publication Year: 2026
Project Info: NanoimmunoEra Project, funded by European Union’s MSCA Staff exchange Horizon Europe programme, Horizon 2020 Programme. Grant Agreement Number 101086341; https://nanoimmunoera-project.eu.

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Dataset Contents
The dataset consists of:
• 4 folders
"Different_MOF_loadings"
"ECL_calibration_curve_at_different_cTnI_concentrations"
"Effect_of_luminophore_concentration"
"Effect_of_PBS_pH"


• 1 README file
"Nano-ImmunoEra_WP2_T2-4_ECL-quantification-of-cTnI-on-SPCE_Cu3-HHTP-2_README.txt"

Dataset Documentation

Abstract
This dataset reports data obtained within the NanoimmunoEra project, Work Package 2 (Production and characterization of materials for improved ECL-based biosensing), specifically Task 2.4 (Electrochemical and ECL performance of ink-jet printed electrodes); Disposable ink-jet printed and screen-printed electrodes were tested to evaluate their influence on electrochemical and ECL performances, with optimization aimed at developing low-cost sensors. 
Early detection of cardiac troponin I (cTnI) demands ultrasensitive electrochemiluminescence (ECL) platforms to enable timely intervention and better prognosis. Here, screen-printed carbon electrodes (SPCEs) were modified by drop-casting Cu₃(HHTP)₂ conductive metal-organic frameworks, yielding a highly responsive sensing substrate that was further engineered with anti-cTnI antibodies and BSA passivation for specific target capture.​
Using [Ru(bpy)₃]²⁺ as luminophore, the Cu₃(HHTP)₂-modified interface generated markedly intensified ECL signals versus bare SPCEs, driven by the material's superior electrical conductivity, extensive porosity, expanded electroactive area, and—most importantly—the ECL-promoting action of HHTP ligands.​
Performance calibration against graded cTnI standards revealed exceptional analytical figures of merit, including an in vitro limit of detection of 10.23 ± 1.06 pg mL⁻¹—far surpassing clinical decision limits. This co-reactant-independent Cu₃(HHTP)₂ ECL immunosensor thus emerges as a promising, high-efficiency tool for swift point-of-care assessment of low-abundance cardiac markers.​

Content of the datasets:

• dataset "Different_MOF_loadings" contains raw ECL intensity data (a.u.) corresponding to five distinct c-MOF loading levels (1, 2, 3, 4, 5 mg/mL);

• dataset "Effect_of_luminophore_concentration" includes ECL intensity measurements (a.u.) with standard deviations for four luminophore concentrations (1, 50, 100, 150 μM);

• dataset "Effect_of_PBS_pH" provides ECL intensity data (a.u.) for five pH conditions (6, 6.5, 7, 7.5, 8);

• dataset "ECL_calibration_curve_at different_cTnI_concentrations" presents ECL intensity values (a.u.) with standard deviations across eight cTnI concentrations (0, 10, 20, 30, 40, 50, 60, 80 pg/mL).
