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README File
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Dataset Title: “Visualizing Interfacial Dynamics and Reaction Kinetics in Electrochemiluminescence via Reflective Impedance Microscopy”.

Dataset Authors: Gabriele Giagu (University of Bologna); 
Alessandro Fracassa (University of Bologna); 
Chiara Pasolini (University of Bologna);
Manuela Iglesias Cernadas (University of Bologna);
Riccardo Pinotti (Universidade da Coruña);
Alejandro Criado (Universidade da Coruña);
Francesco Paolucci (University of Bologna);
Giovanni Valenti (University of Bologna).

Dataset Contact Person: Giovanni Valenti (University of Bologna), g.valenti@unibo.it; Francesco Paolucci (University of Bologna), francesco.paolucci@unibo.it.

Dataset License: this dataset is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/.

Publication Year: 2026

Project Info: SENSOPAD project, funded by the European Union’s Horizon Europe Research and Innovation Programme under grant agreement n° 101130516.


Dataset Contents
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The dataset consists of a .zip archive "RIM_ECL_Interfacial_Dynamics.zip" containing the following files and folders

RIM_ECL_Interfacial_Dynamics.zip
└── Bead_Diffusion_Dynamics
    ├── Data
    │   ├── Tiff
    │   │   ├── Elab_Opt.jpg
    │   │   ├── ss_single_<0-100>.tiff
    │   └── txt
    │       └── LASV_400mV_C01.txt
    Calibration_Data
    ├── Data
    │   ├── Tiff
    │   │   ├── 100mV
    │   │   ├── ss_single_<0-100>.tiff
    │   │   ├── 200mV
    │   │   ├── ss_single_<0-100>.tiff
    │   │   ├── 300mV
    │   │   ├── ss_single_<0-100>.tiff
    │   │   ├── 400mV
    │   │   ├── ss_single_<0-100>.tiff
    │   │   ├── 500mV
    │   │   ├── ss_single_<0-100>.tiff
    │   │   └── Elab_Opt.jpg
    │   └── txt
    │       ├── LASV_100mV_C01.txt
    │       ├── LASV_200mV_C01.txt
    │       ├── LASV_300mV_C01.txt
    │       ├── LASV_400mV_C01.txt
    │       └── LASV_500mV_C01.txt
    Graphene_Reaction_Kinetics
    ├── Data
    │   ├── Current_EB_CA_ECL_1,4V_2+8s_Trans_Sens200_Gain5_03_CA_C01.txt
    │   ├── EB_CA_ECL_1,4V_2+8s_Trans_Sens200_Gain5
    │   │   ├── Fig1_<1-51>.tif
    │   └── opt_EB_CA_ECL_1,4V_2+8s_Trans_Sens200_Gain5.tif
    RIM_ECL_Interfacial_Dynamics_README.txt
    


Abstract
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This dataset was produced as part of the SENSOPAD project and contains synchronized optical and electrical measurements obtained via a Reflective Impedance Microscopy (RIM) and Electrochemiluminescence Microscopy (ECLM) imaging platform. It includes Large Amplitude Sinusoidal Voltammetry (LASV) electrochemical data and corresponding high-framerate optical TIFF sequences used to spatially resolve the impedance of the electrode-solution interface. 
The dataset contains measurements of bead-environment diffusion dynamics using the Fe(CN)63-\4- redox couple, and mapping of TPrA oxidation kinetics on graphene-modified gold electrodes. 
Additionally, it provides the 2D axisymmetric finite element simulation files (COMSOL) used to evaluate coupled mass transport and electrode reactions around a single microbead. Together, these data support the reproducibility of the RIM analysis and provide orthogonal insights into the mechanisms involved in ECL signal generation.

Content of the folders
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"Calibration_Data"
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The folder "Calibration_Data" contains the synchronized TIFF image stacks and EC-Lab text exports (.mpt/.txt) required to calculate the calibration proportionality factor ($\beta$).

This folder contains the raw data required to calculate the calibration proportionality factor (beta) used in the Reflective Impedance Microscopy (RIM) analysis[cite: 1, 3]. 

Folder Contents:
* High-framerate optical image stacks (.tif or .tiff files)[cite: 1, 3].
* EC-Lab software text exports (.mpt or .txt files) containing synchronized electrochemical measurements[cite: 1, 3].

Usage:
These data are intended for use with the "Calibration Mode" module of the RIM Analysis Suite.

The folder consists of:
* 500 captured image frames
* "ss_single_1.tif" - "ss_single_100.tif" (For Each Folder at 100mV-200mV-300mV-400mV-500mV)
* 5 EC-Lab file
* "LASV_100mV_C01-LASV_200mV_C01-LASV_300mV_C01-LASV_400mV_C01-LASV_500mV_C01"
* 1 optical image of the electrode
* "Elab_Opt.tif"
* 1 Readme file
* "README_CalibrationData.txt"

This folder contains synchronized optical and electrochemical data acquired from Reflective Impedance Microscopy (RIM) experiments to map the diffusion dynamics of the Fe(CN)6 3-/4- redox couple in the presence of 12 μm magnetic microbeads. The dataset is intended to validate the boundary effect exerted by the beads on the diffusion layer.

The experimental conditions of the RIM measurements were:
* Analyte: 10 mM solution-phase Fe(CN)6 3-/4-[cite: 3]
* Electrolyte: 0.3 M phosphate buffer, pH 6.8[cite: 3]
* Electrode Configuration: Gold disk working electrode with drop-cast 12 μm magnetic microbeads, Platinum wire counter electrode, and Ag/AgCl reference electrode.
* Electrochemical Protocol: Large Amplitude Sinusoidal Voltammetry (LASV)[cite: 3].
* Temperature: Room temperature
* Recording parameters: CMOS camera (Teledyne CMOS 01-MOMENT-MONO) mounted on an inverted microscope (Nikon Eclipse Ti2-E)[cite: 3]. 512x512 pixels - exposure time 0,0048 s .

Content of the files
* "ss_single_1.tif" - "ss_single_100.tif" contains 32-bit floating-point TIFF image frames of the optical reflectivity recorded during the LASV experiment.
* "LASV_100mV_C01-LASV_200mV_C01-LASV_300mV_C01-LASV_400mV_C01-LASV_500mV_C01" contains the electrochemical data (Time, Applied Potential, and Current) synchronized with the optical frames.


"Bead_Diffusion_Dynamics"
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This folder contains the raw experimental data for mapping and analyzing diffusion dynamics at the electrode-solution interface in the presence of a spherical boundary.

Experimental Conditions:
* Solution: 0.3 M phosphate buffer (PB) at pH 6.8.
* Redox Probe: 10 mM of the Fe(CN)6 3-/4- redox couple.
* Surface: Gold electrodes modified with 12 μm magnetic microbeads.

Folder Contents:
* Raw TIFF files containing the RIM optical sequences[cite: 1, 3].
* Associated LASV (Large Amplitude Sinusoidal Voltammetry) electrochemical files.

Usage:
These data can be processed using the "Measurement Mode" and "Real & Imaginary Mapping" modules of the RIM Analysis Suite to generate spatially resolved impedance maps.

Folder "Bead_Diffusion_Dynamics" contains raw RIM measurements (TIFF and LASV files) conducted in a 0.3 M phosphate buffer solution (pH 6.8) containing 10 mM of the Fe(CN)63-\4- redox couple on electrodes modified with 12 μm magnetic microbeads.

The folder consists of:
* 100 captured image frames
* "ss_single_1.tif" - "ss_single_100.tif" 
* 1 EC-Lab file
* "LASV_400mV_C01"
* 1 optical image of the electrode
* "Elab_Opt.tif"
* 1 Readme file
* "README_BeadDiffusionDynamics.txt"

This folder contains synchronized optical and electrochemical data acquired from Reflective Impedance Microscopy (RIM) experiments to map the diffusion dynamics of the Fe(CN)6 3-/4- redox couple in the presence of 12 μm magnetic microbeads. The dataset is intended to validate the boundary effect exerted by the beads on the diffusion layer.

The experimental conditions of the RIM measurements were:
* Analyte: 10 mM solution-phase Fe(CN)6 3-/4-
* Electrolyte: 0.3 M phosphate buffer, pH 6.8
* Electrode Configuration: Gold disk working electrode with drop-cast 12 μm magnetic microbeads, Platinum wire counter electrode, and Ag/AgCl reference electrode.
* Electrochemical Protocol: Large Amplitude Sinusoidal Voltammetry (LASV).
* Temperature: Room temperature
* Recording parameters: CMOS camera (Teledyne CMOS 01-MOMENT-MONO) mounted on an inverted microscope (Nikon Eclipse Ti2-E). 512x512 pixels - exposure time 0,0048 s .

Content of the files
* "ss_single_1.tif" - "ss_single_100.tif" contains 32-bit floating-point TIFF image frames of the optical reflectivity recorded during the LASV experiment.
* "C01-LASV_400mV_C01" contains the electrochemical data (Time, Applied Potential, and Current) synchronized with the optical frames.


"Graphene_Reaction_Kinetics"
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Folder "Graphene_Reaction_Kinetics" contains RIM and ECLM raw measurements acquired in 0.3 M PB (pH 6.8) containing 50 mM TPrA on gold electrodes modified with graphene deposits and Ru(II)-functionalized beads.

Description:
This folder contains the raw measurements used to visualize and quantify the differences in oxidation kinetics between the bare gold electrode surface and graphene deposits.

Experimental Conditions:
* Solution: 0.3 M phosphate buffer (PB) at pH 6.8.
* Coreactant: 50 mM TPrA (Tri-n-propylamine).
* Surface: Gold electrodes modified with graphene deposits and Ru(II)-functionalized beads.

Folder Contents:
* Raw ECLM (Electrochemiluminescence Microscopy) data for emission mapping.

Folder "Graphene_Reaction_Kinetics_ECLM" contents

The folder consists of:
* 51 captured image frames
* "Fig1_1.tif" - "Fig1_51.tif" 
* 1 txt file
* "Current_EB_CA_ECL_1,4V_2+8s_Trans_Sens200_Gain5_03_CA_C01.txt"
* 1 Oprical image
* "opt_EB_CA_ECL_1,4V_2+8s_Trans_Sens200_Gain5.tif"
* 1 Readme file
* "README_Graphene_Reaction_Kinetics_ECLM.txt"

This folder contains synchronized optical and electrochemical data acquired from Electrochemiluminescence Microscopy (ECLM) experiments to visualize and map the TPrA oxidation kinetics and subsequent ECL emission on gold electrodes modified with graphene deposits and Ru(II)-functionalized magnetic microbeads. The dataset is intended to evaluate the localized enhancement of the ECL emission due to the faster electron transfer kinetics of the graphene deposits.

The experimental conditions of the ECLM measurements were:
* Coreactant: 50 mM solution-phase Tri-n-propylamine (TPrA)
* Electrolyte: 0.3 M phosphate buffer, pH 6.8[cite: 2, 3]
* Electrode Configuration: Gold disk working electrode with drop-cast graphene particles and Ru(II)-functionalized magnetic microbeads, Platinum wire counter electrode, and Ag/AgCl reference electrode.
* Electrochemical Protocol: Chronoamperometry (Open circuit potential for 2 s, followed by 1.4 V vs Ag/AgCl for 8 s).
* Temperature: Room temperature
* Recording parameters: Ultrasensitive EM-CCD camera (EM-CCD 9100-13, Hamamatsu, Japan) mounted on a Nikon Eclipse Ti2-E inverted microscope. Enclosed in a custom-built dark box. Nikon air objective (magnification 100×/NA 0.8/WD 4.5 mm). Resolution: 512x512 pixels. Images captured at 200 ms intervals. Sensitivity 200 Gain 5.

Content of the files
* "ecl_single_1.tif" - "ecl_single_[XXXX].tif" contains TIFF image frames of the ECL emission recorded during the chronoamperometric pulse[cite: 2, 3].
* "Current_EB_CA_ECL_1,4V_2+8s_Trans_Sens200_Gain5_03_CA_C01.txt" contains the electrochemical data (Time, Applied Potential, and Current) synchronized with the optical frames.
