README file
Data Set Title: “Dataset of cats with calicivirus infection”
Data Set Author/s: 
Magliocca Martina (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);
Balboni Andrea (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);
Mandrioli Luciana (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);
Bacci Barbara (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);
Ballotta Giulia (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);
Scarpellini Raffaele (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);
Battilani Mara (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna);

Data Set Contact Person/s: Magliocca Martina (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna), email: martina.magliocca2@unibo.it; Balboni Andrea (Department of Veterinary Medical Science, Alma Mater Studiorum – University of Bologna), email: a.balboni@unibo.it

Data Set License: this data set is distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, https://creativecommons.org/licenses/by/4.0/

Publication Year: 2025
Project Info: COVDC (Exploring the virome of domestic dogs and cats COVDC (Comprehending virome of domestic carnivores): exploring the viral diversity of domestic carnivores and One Health implications), funded by European Union - European Union - Next-GenerationEU - National Recovery and Resilience Plan (NRRP) – MISSION 4 COMPONENT C2, INVESTIMENT N. 1.1, CALL PRIN 2022 PNRR D.D. 1409 14-09-2022. Project number P2022FR49N. CUP N. J53D23014340001. https://www.uniba.it/it/ricerca/dipartimenti/dipmedveterinaria/ricerca/progetti-1/progetti-prin-2022-pnrr/cod-p2022fr49n-exploring-the-virome-of-domestic-dogs-and-cats-covdc-comprehending-virome-of-domestic-carnivores-exploring-the-viral-diversity-of-domestic-carnivores-and-one-health-implications
Data set Contents
The data set consists of:
•	1 tabular qualitative file in .xlsx format
COVDC_DatasetCatCalicivirusInfection_2025.xlsx
•	1 readme file in rtf format
COVDC_DatasetCatCalicivirusInfection_2025_README.rtf

Data set Documentation
Abstract
The dataset contains signalament data, clinical signs and diagnostic data of a litter composed by a female adult cat and four 20-days old kitten, presented to the Veterinary Teaching Hospital of the Department of Veterinary Medical Science of the University of Bologna, in which an infection of feline calicivirus is reported.
Content of the files: 
•	file COVDC_DatasetCatCalicivirusInfection_2025_xlsx contains signalament data, clinical signs and diagnostic data of a litter composed by a female adult cat and three 20-days old kitten, presented to the Veterinary Teaching Hospital of the Department of Veterinary Medical Science of the University of Bologna, in which an infection of feline calicivirus is reported. Specifically, information about the breed, the origin, the age, the sex, the vaccination status, the presence of clinical signs, the outcome, and laboratory investigastion are indicated for each cat. Post-mortem examination, samples collected, histopathological, immuhistochemical and bacteriological analysis, RT-qPCR are carried out only on one cat. 
Methodologies 
The presence of FCV viral RNA was detected using a specific SYBR Green real-time qPCR (qPCR) assay with primers qFCVf (5’-TAA TTC GGT GTT TGA TTT GGC CTG GGC T-3’) and qFCVr (5’-CAT ATG CGG CTC TGA TGG CTT GAA ACT G-3’) amplifying a fragment of 83 nucleotides (nts) of the ORF1 region (Helps et al., 2002)1. The Power SYBR Green RNA-to-CT 1-Step kit (Life Technologies, California, USA) was used in a total volume of 20 μL, following the manufacturer’s instruction. Serial 10-fold dilutions of a plasmid (pCR4 plasmid, TOPO TA Cloning Kit, Life Technologies, USA) containing one copy of the target sequence were used as external standards for the construction of standard curve. The melting experiment for the evaluation of the specificity of each reaction was performed after the last extension step by a continuous increment from 60°C to 95°C. Specific melting temperature was about 81°C and the limit of detection (LOD) of the assay was one copy of target amplicon/µL of RNA extract (copies/µL).

1.	Helps C.; Lait P.; Tasker S.; Harbour D. Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR. J. Virol. Methods 2002, 106, 241–244. https://doi.org/10.1016/S0166-0934(02)00167-2