README file

Dataset Title: “Quantitative data on immune cell infiltration (T lymphocytes, B lymphocytes, and macrophages) in canine soft tissue sarcomas.”
Dataset Author/s: 
•	Giancarlo Avallone (University of Bologna, Department of Veterinary Medical Sciences – DIMEVET, Bologna, Italy), ORCID 0000-0002-5203-7679;
•	Elena Brigandì (University of Bologna, Department of Veterinary Medical Sciences – DIMEVET, Bologna, Italy); 
•	Barbara Bacci (University of Bologna, Department of Veterinary Medical Sciences – DIMEVET, Bologna, Italy), ORCID 0000-0002-2206-0384;
•	Antonella Rigillo (I-Vet, Brescia, Italy)
•	Paola Roccabianca (Università Degli Studi di Milano, Department of Veterinary Medicine and Animal Sciences, Lodi, Italy) ORCID 0000-0002-3672-4612;
•	Chiara Tugnoli (University of Bologna, Department of Veterinary Medical Sciences – DIMEVET, Bologna, Italy); 

Data Set Contact Person: Giancarlo Avallone (University of Bologna, Department of Veterinary Medical Sciences – DIMEVET, Bologna, Italy), ORCID 0000-0002-5203-7679;             giancarlo.avallone@unibo.it
Data Set License: this data set is distributed under a Creative Commons CC0 1.0 Universal (CC0 1.0) license, https://creativecommons.org/publicdomain/zero/1.0/  
Publication Year: 2026
Data set Contents
The data set consists of:
•	1 tabular quantitative file saved in .csv format “Canine_STS_immunecells.csv”
•	1 README file “Canine_STS_immunecells_README.rtf”
Abstract
The dataset contains the quantitative data regarding immune cell infiltration in a case series of canine soft tissue sarcoma. Immune cells were quantified by immunohistochemistry followed by image analysis on digitalised sections. Markers used to detect immune cells were: CD20 (B cells), CD3 (T cells), FOXP3, (Treg cells), Iba-1 (macrophages), CD204 (M2-like macrophages). For each case the age, breed and sex of the affected animal were also collected. Histological variables included in the datasets are the specific diagnosis (sarcoma type), mitotic count and histological grade.
Content of the files:
•	file “Canine_STS_immunecells.csv” contains all the variables listed below
List of variables
CD20+ cell density (cells/mm²): number of cells staining positive for CD20, in 1 mm²
CD3+ cell density (cells/mm²): number of cells staining positive for CD3, in 1 mm²
FOXP3+cell density (cells/mm²): number of cells staining positive for CD20, in 1 mm²
Treg proportion (as % of T cells): % of T cells expressing FOXP3
B/T ratio: ratio between CD20+cell density and CD3+cell density
Total TILS/mm²: number of cells staining positive for CD20 and cells staining positive for CD3, in 1 mm2
Iba-1 score: percentage of tumor area staining positive for Iba-1
CD204 score: percentage of tumor area staining positive for CD204
Iba-1/CD204 score: ratio between Iba-1 score and CD204 score
Mitotic count in 2,37mm²: number of mitotic figures counted in 2,37 mm² in the less differentiated and more cellular portion of the neoplasm
Histological grade: histological grade calculated as reported in the literature (Avallone G, et al. Review of Histological Grading Systems in Veterinary Medicine. Vet Pathol. 2021 Sep;58(5):809-828. doi: 10.1177/0300985821999831.)	
Age (years): age of the affected dog at the diagnosis
Sex: sex of the affected dog
Breed: breed of the affected dog
Notes
The data are presented in the following publication:
•	Avallone G, et al. Tumor-infiltrating lymphocytes vary in different canine soft tissue sarcoma histological types. Vet Pathol. 2025 May;62(3):276-283. doi: 10.1177/03009858241300556. 
•	Avallone G, et al. Characterization of tumor-associated macrophages in canine soft tissue sarcomas reveals histotype-dependent immune microenvironments and correlations with mitotic count and histological grade. Vet Pathol. 2026 Jan 11:3009858251409221. doi: 10.1177/03009858251409221.