ConcePTION WP3 task 3.2. Isolation of porcine Mammary Epithelial Cells (pMECs). In vitro model for epithelial barrier

Bernardini, Chiara ; La Mantia, Debora ; Salaroli, Roberta ; Zannoni, Augusta ; Nauwelaerts, Nina ; Deferm, Neel ; Ventrella, Domenico ; Bacci, Maria Laura ; Sarli, Giuseppe ; Bouisset-Leonard, Michele ; Annaert, Pieter ; Forni, Monica (2021) ConcePTION WP3 task 3.2. Isolation of porcine Mammary Epithelial Cells (pMECs). In vitro model for epithelial barrier. University of Bologna. DOI 10.6092/unibo/amsacta/6660. [Dataset]
Full text disponibile come:
[thumbnail of README file] Documento di testo(rtf) (README file)
Licenza: Creative Commons: Attribuzione 4.0(CC BY 4.0)

Download (694kB)
[thumbnail of Data set] Archivio (Data set)
Licenza: Creative Commons: Attribuzione 4.0(CC BY 4.0)

Download (128kB)

Abstract

The present research aimed to develop a method for the isolation and culture of porcine Mammary Epithelial Cells (pMECs) to study mammary epithelial barrier in vitro. In agreement with the application of the 3Rs, porcine mammary gland tissues were collected at a local slaughterhouse and, after dissociation, the cells were isolated, cultured and characterized by morphology, cell cycle analysis and immunophenotyping. The ability to create a barrier was tested by TEER (Transepithelial/transendothelial electrical resistance) and the gene expression of genes related to drug transporters was evaluated by a PCR array. All the three pMECs cellular lines isolated are able to create a tight barrier and express the main ABC and SLC drug transporters confirming that the method of isolation is efficient to obtain primary pMECs lines to study epithelial barrier functions in the pig model. This activity was carried out as a part of Conception project (GA 821520).

Abstract
Tipologia del documento
Dataset
Autori
AutoreAffiliazioneORCID
Bernardini, ChiaraUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0003-3384-0244
La Mantia, DeboraUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0002-5649-1852
Salaroli, RobertaUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0002-8819-2857
Zannoni, AugustaUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0002-2972-4921
Nauwelaerts, NinaKU Leuven0000-0001-5301-6813
Deferm, NeelKU Leuven0000-0002-5014-1691
Ventrella, DomenicoUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0002-9330-979X
Bacci, Maria LauraUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0001-6702-5868
Sarli, GiuseppeUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0001-6405-3484
Bouisset-Leonard, MicheleNovartis Pharma AG - Novartis Institutes for BioMedical Research
Annaert, PieterKU Leuven0000-0003-3525-7351
Forni, MonicaUniversity of Bologna - Dept. of Veterinary Medical Sciences0000-0003-1310-6202
Settori scientifico-disciplinari
DOI
Contributors
Contributor
Affiliazione
ORCID
Tipo
Zannoni, Augusta
University of Bologna - Dept. of Veterinary Medical Sciences
Contact person
Data di deposito
23 Apr 2021 15:11
Ultima modifica
19 Mag 2023 11:33
Nome del Progetto
ConcePTION - Building an ecosystem for better monitoring and communicating of medication safety in pregnancy and breastfeeding: validated and regulatory endorsed workflows for fast, optimised evidence generation
Programma di finanziamento
EC - H2020
URI

Altri metadati

Statistica sui download

Statistica sui download

Gestione del documento: Visualizza il documento

^