Peptide Fractions Obtained from Rice By- Products by Means of an Environment- Friendly Process Show In Vitro Health-Related Bioactivities

Ferri, Maura ; Graen-Heedfeld, Jurgen ; Bretz, Karlheinz ; Guillon, Fabien ; Elisa, Michelini ; Calabretta, Maria Maddalena ; Lamborghini, Matteo ; Gruarin, Nicolò ; Roda, Aldo ; Kraft, Axel ; Tassoni, Annalisa (2017) Peptide Fractions Obtained from Rice By- Products by Means of an Environment- Friendly Process Show In Vitro Health-Related Bioactivities. PLOS ONE, 12 (1).
Full text available as:
[thumbnail of Ferri et al PLOS ONE 2017.pdf]
License: Creative Commons: Attribution 4.0 (CC BY 4.0)

Download (1MB) | Preview


Recently, the isolation of new health-related bioactive molecules derived from agro-food industrial by-products by means of environment-friendly extraction processes has become of particular interest. In the present study, a protein by-product from the rice starch industry was hydrolysed with five commercial proteolytic enzymes, avoiding the use of solvents or chemicals. The digestion processes were optimised, and the digestates were separated in fractions with four different molecular weight ranges by using a cross-flow membrane filtration technique. Total hydrolysates and fractions were tested in vitro for a wide range of biological activities. For the first time rice-derived peptides were assayed for anti-tyrosinase, anti-inflammatory, cytotoxicity and irritation capacities. Antioxidant and anti-hypertensive activities were also evaluated. Protamex, Alcalase and Neutrase treatments produced peptide fractions with valuable bioactivities without resulting cytotoxic or irritant. Highest levels of bioactivity were detected in Protamex-derived samples, followed by samples treated with Alcalase. Based on the present results, a future direct exploitation of isolated peptide fractions in the nutraceutical, functional food and cosmetic industrial fields may be foreseen.

Document type
Ferri, MauraUniversity of Bologna, Italy
Graen-Heedfeld, JurgenFraunhofer Institute, Germany
Bretz, KarlheinzFraunhofer Institute, Germany
Guillon, FabienSterlab, France
Elisa, MicheliniUniversity of Bologna, Italy
Calabretta, Maria MaddalenaUniversity of Bologna, Italy
Lamborghini, MatteoCarminia, Italy
Gruarin, NicolòUniversity of Bologna, Italy
Roda, AldoUniversity of Bologna, Italy
Kraft, AxelFraunhofer Institute, Germany
Tassoni, AnnalisaUniversity of Bologna, Italy
peptodis, rice, anti-oxidant activity, enzymes
Deposit date
21 Feb 2017 11:17
Last modified
08 May 2017 13:05
Project name
BIORICE - BIOtechnology for the recovery of valuable peptides from industrial RICE by-products and production of added value ingredients for nutraceuticals, functional foods and cosmetics
Funding program
EC - FP7

Other metadata



Staff only: View the document