SPARTEVs. Gene expression data of cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes

Mateo-Otero, Yentel ; Yeste, Marc ; Roca, Jordi ; Llavanera, Marc ; Bucci, Diego ; Galeati, Giovanna ; Spinaci, Marcella ; Barranco, Isabel (2022) SPARTEVs. Gene expression data of cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes. University of Bologna. DOI 10.6092/unibo/amsacta/7138. [Dataset]
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Extracellular vesicles (EVs), nano-sized (30-to-1000 nm) membrane vesicles secreted by all functional cells, are essential intercellular mediators, modulating several physiological and pathological processes. According to their size and biogenic pathway, EVs released by living cells are classically categorized into exosomes (small EVs) and microvesicles (large EVs). EVs has been isolated from all biofluids, including seminal plasma (SP), a fluid composed by secretions from accessory sex glands that accompanies sperm during and after ejaculation. Seminal EVs modulate several sperm physiological processes, and female reproductive tract immune environment, which is required for successful embryo development. Evidence supports that SP may also affect ovarian function. This study evaluated the potential effect of supplementing with two SP-EVs subsets (small (S-EVs) and large (L-EVs)) during in vitro maturation (IVM) of porcine oocytes on gene expression of cumulus cells. Four pathways were investigated using a total of nine genes: i) cell apoptosis using B-cell lymphoma 2 (BCL2) and BCL2 Associated X (BAX); ii) cell proliferation using Cyclin B1 (CCNB1); iii) oocyte maturation through Connexin 43 (CX43), Hyaluronan Synthase 2 (HAS2) and Stearoyl-CoA desaturase 1 (SCD1); and iv) steroidogenesis using Cytochrome P450 Family 11 Subfamily A Member 1 (CYP11A1), Hydroxy-Delta-5-Steroid Dehydrogenase 3 Beta (HSD3B1) and aromatase (CYP19A1). All genes, including housekeeping 60S Ribosomal Protein L18 (RPL19), were selected based on previous literature. The mRNA levels were assessed by quantitative real-time PCR.

Document type
Mateo-Otero, YentelUniversity of Girona, Spain0000-0002-5810-211X
Yeste, MarcUniversity of Girona, Spain0000-0002-2209-340X
Roca, JordiUniversity of Murcia, Spain0000-0002-4213-3093
Llavanera, MarcUniversity of Girona, Spain0000-0003-0685-7534
Bucci, DiegoUniversity of Bologna, Italy0000-0001-6276-2674
Galeati, GiovannaUniversity of Bologna, Italy0000-0001-5149-108X
Spinaci, MarcellaUniversity of Bologna, Italy0000-0002-8934-9035
Barranco, IsabelUniversity of Bologna, Italy0000-0001-9873-814X
seminal plasma, extracellular vesicles, oocyte, cumulus cells, gene expression, in vitro maturation
Barranco, Isabel
University of Bologna, Italy
Contact person
Bucci, Diego
University of Bologna, Italy
Contact person
Deposit date
09 Jan 2023 15:17
Last modified
09 Jan 2023 15:17
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Relation type
this upload is supplement to
Project name
SPARTEVs - Impact of extracellular vesicles isolated from seminal plasma upon Assisted Reproductive Technology (oocyte maturation, fertilization and embryo culture) in a mammalian model
Funding program
EC - H2020

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