Abstract

The wide use of glyphosate-based herbicides (GBHs) has become a matter of concern due to its potential harmful effects on human health, including men fertility. The study investigated, using the pig as a model, the impact of pure glyphosate and its most known commercial formulation, Roundup, on sperm function and survival. These data sets contain the raw data of the flow cytometry analysis of sperm parameters (sperm viability, acrosome integrity, mitochondrial activity and DNA fragmentation) and motility analysis by computer-assisted sperm analysis (CASA) of fresh commercial semen doses incubated with different concentrations (0–360 μg/mL) of glyphosate (GLY) or Roundup (R), at the equivalent GLY concentration for 3 h.