Abstract

The current global plastic pollution of all ecosystems allows the accumulation of micro- and nanoplastics (MNPs) in human and animal tissues. These particles have been associated with negative effects on fertility, primarily through inflammation and oxidative stress in reproductive organs. Given the limited research on how MNPs affect gametes, this study aimed to evaluate MNPs internalization and related effects in mature spermatozoa, using frozen-thawed stallion sperm exposed to various concentrations of 30 nm polystyrene nanoplastics (NP). The datasets include flow cytometry data on sperm viability, intracellular reactive oxygen species levels, mitochondrial membrane potential, mean fluorescence intensity (MFI) of H2DCFDA, and mean fluorescence intensity (MFI) of fluorescent nanoplastics (FNP). Additionally, they include kinetic sperm parameters analyzed by computer-assisted sperm analysis (CASA). Semen samples were incubated for 3 hours at 38 ºC with or without 30 nm NP at 10, 50, 100 or 200 µg/mL.