Effects of hydrogen peroxide and glutathione on recombinant beta-amylase1 (BAM1) from Arabidopsis thaliana and its behavior in plants with altered glutathione homeostasis

Gurrieri, Libero ; Capuzzi, Anna Clara ; Müller-Schüssele, Stefanie ; Trost, Paolo ; Sparla, Francesca (2025) Effects of hydrogen peroxide and glutathione on recombinant beta-amylase1 (BAM1) from Arabidopsis thaliana and its behavior in plants with altered glutathione homeostasis. University of Bologna. DOI 10.6092/unibo/amsacta/8477. [Dataset]
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Abstract

The present dataset contains redox treatments of BAM1 with redox reagents like hydrogen peroxide and glutathione. The effect of glutathione has been monitored at several timepoints. Release of glutathione from BAM1 has been monitored and quantified. The inhibition following the release of glutathione has been detailed performing reactivations with thioredoxins, glutathioredoxins, dithiothreitol and glutathione. Arabidopsis thaliana plants with low levels of glutathione reductase 2 in chloroplasts were analyzed by in-gel amylolytic activity. In the same genotypes starch granule area in guard cells and stomata aperture have been quantified.

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Gurrieri, Libero0000-0002-3537-2512Department of Pharmacy and Biotechnology, University of Bologna, Italy
Capuzzi, Anna Clara0009-0004-3376-2148Department of Pharmacy and Biotechnology, University of Bologna, Italy
Müller-Schüssele, Stefanie0000-0003-4061-1175Department of Biology, University of Kaiserslautern-Landau, Germany
Trost, Paolo0000-0002-6347-8701Department of Pharmacy and Biotechnology, University of Bologna, Italy
Sparla, Francesca0000-0002-2103-4870Department of Pharmacy and Biotechnology, University of Bologna, Italy
Keywords
beta-amylase arabidopsis starch glutathione redox regulation
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Gurrieri, Libero
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Department of Pharmacy and Biotechnology, University of Bologna, Italy
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25 Aug 2025 08:16
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25 Aug 2025 08:16
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