Abstract

cAMP has been reported to be an essential driver of sperm capacitation and cAMP efflux through multidrug resistance-associated protein 4 (MRP4) has been suggested to maintain intracellular cAMP homeostasis and generate extracellular signaling able to regulate capacitation. Using an in vitro model of pig sperm capacitation, this study investigated the influence of extracellular cAMP on sperm capacitation and acquisition of fertilizing ability, and evaluated the role of MRP4 using MK571, a MRP4 selective inhibitor. These data sets contain the raw data on sperm tyrosine-phosphorylated protein localization, sperm viability and in vitro fertilization (IVF) output.