Abstract

Research on extracellular vesicles (EVs), membrane-delimited nanoparticles (30-to-1000 nm) released by functional cells is currently a challenge for scientific community since they act as essential messengers in pathological and physiological processes, including those related to reproduction. Based on differences in size and biogenesis pathways, EVs released by healthy living cells are classically categorized into exosomes (small EVs) and microvesicles (large EVs). These EVs has been isolated from all reproductive fluids, including seminal plasma (SP), a complex and heterogeneous fluid composed mainly by secretions from accessory sexual glands that accompanies sperm during and after ejaculation. Bearing in mind that SP is beneficial for sperm-oocyte interaction and embryo development and that it contains EVs, which are involved in several sperm functions, the hypothesis of SPARTEVs project was that seminal EVs may play a key role in the in the successful development of the above-mentioned reproductive events. This data set contains the raw data of the characterization of EVs subsets (small (S-EVs) and large (L-EVs)) isolated from pig SP (by a combination of centrifugation, size-exclusion chromatography and ultrafiltration) and used for supplementing in vitro maturation medium of porcine oocytes. The characterization was conducted following MISEV2018 guidelines, and this data set included the raw data obtained from dynamic scattering, nano tracking particle and flow cytometry analyses.