SPARTEVs. Characterization of seminal extracellular vesicles subsets used for supplementing in vitro maturation medium of porcine oocytes

Mateo-Otero, Yentel ; Yeste, Marc ; Roca, Jordi ; Llavanera, Marc ; Bucci, Diego ; Galeati, Giovanna ; Spinaci, Marcella ; Barranco, Isabel (2022) SPARTEVs. Characterization of seminal extracellular vesicles subsets used for supplementing in vitro maturation medium of porcine oocytes. University of Bologna. DOI 10.6092/unibo/amsacta/7124. [Dataset]
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Abstract

Research on extracellular vesicles (EVs), membrane-delimited nanoparticles (30-to-1000 nm) released by functional cells is currently a challenge for scientific community since they act as essential messengers in pathological and physiological processes, including those related to reproduction. Based on differences in size and biogenesis pathways, EVs released by healthy living cells are classically categorized into exosomes (small EVs) and microvesicles (large EVs). These EVs has been isolated from all reproductive fluids, including seminal plasma (SP), a complex and heterogeneous fluid composed mainly by secretions from accessory sexual glands that accompanies sperm during and after ejaculation. Bearing in mind that SP is beneficial for sperm-oocyte interaction and embryo development and that it contains EVs, which are involved in several sperm functions, the hypothesis of SPARTEVs project was that seminal EVs may play a key role in the in the successful development of the above-mentioned reproductive events. This data set contains the raw data of the characterization of EVs subsets (small (S-EVs) and large (L-EVs)) isolated from pig SP (by a combination of centrifugation, size-exclusion chromatography and ultrafiltration) and used for supplementing in vitro maturation medium of porcine oocytes. The characterization was conducted following MISEV2018 guidelines, and this data set included the raw data obtained from dynamic scattering, nano tracking particle and flow cytometry analyses.

Abstract
Document type
Dataset
Creators
CreatorsAffiliationORCID
Mateo-Otero, YentelUniversity of Girona, Spain0000-0002-5810-211X
Yeste, MarcUniversity of Girona, Spain0000-0002-2209-340X
Roca, JordiUniversity of Murcia, Spain0000-0002-4213-3093
Llavanera, MarcUniversity of Girona, Spain0000-0003-0685-7534
Bucci, DiegoUniversity of Bologna, Italy0000-0001-6276-2674
Galeati, GiovannaUniversity of Bologna, Italy0000-0001-5149-108X
Spinaci, MarcellaUniversity of Bologna, Italy0000-0002-8934-9035
Barranco, IsabelUniversity of Bologna, Italy0000-0001-9873-814X
Keywords
seminal plasma, extracellular vesicles subsets, size-exclusion chromatography, extracellular vesicles isolation, microvesicles, exosomes
Subjects
DOI
Contributors
Name
Affiliation
ORCID
Type
Barranco, Isabel
University of Bologna, Italy
Contact person
Bucci, Diego
University of Bologna, Italy
Contact person
Deposit date
22 Dec 2022 14:01
Last modified
22 Dec 2022 14:01
Project name
SPARTEVs - Impact of extracellular vesicles isolated from seminal plasma upon Assisted Reproductive Technology (oocyte maturation, fertilization and embryo culture) in a mammalian model
Funding program
EC - H2020
URI

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