Abstract

The presented dataset contains data and images produced in the framework of the Horizon Europe Nano-immunoERA project, regarding identification, selection and testing of antigen-nucleic acid (NA) chimera probe for antibody detection.Here, we show that Toehold Switch DNA hairpins, presenting a rationally designed locked protospacer adjacent motif (PAM) in the loop, can be used to control Cas12a in response to molecular inputs inclouding IgG antibodies.Reconfiguring the Toehold Switch DNA from a hairpin to a duplex conformation through a strand displacement reaction provides an effective means to modulate the accessibility of the PAM, thereby controlling the binding and cleavage activities of Cas12a.Through this approach, we showcase the potential to trigger downstream Cas12a activity by leveraging proximity-based strand displacement reactions in response to target binding.Our system enables rapid, one-pot detection of IgG antibodies and small molecules with high sensitivity and specificity