FurNAP: Iron-dependent chromatin conformation by the Ferric Uptake Regulator

Danielli, Alberto (2024) FurNAP: Iron-dependent chromatin conformation by the Ferric Uptake Regulator. University of Bologna. DOI 10.6092/unibo/amsacta/7732. [Dataset]
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Abstract

This dataset contains raw and analyzed data for the DNase Footprinting, RT-qPCR, chromatin conformation capture (3C) and Atomic Force Microscopy (AFM) experiments demonstrating the involvement of the Fur regulator in the iron-mediated control of Helicobacter pylori chromatin contacts. The dataset supports evidence that an orthologue of the widespread Fur regulator family behaves like an iron-dependent Nulecoid Associated Protein (NAP) to dynamically shape the H. pylori chromatin in response to iron repletion. This behavior is consistent with the current holo-Fur DNA binding model, in which the iron co-factor stimulates Fur multimerization and DNA binding. The data strengthen the emerging picture that DNA binding sites of multimeric transcription factors, even when not directly involved in gene regulation, may encompass structural roles in bacterial nucleoid organization. Moreover, as many Fur orthologues seem to exhibit features typical of NAPs, the data also suggest that metal-dependent chromatin interactions may be widespread in the bacterial domain, with important implications in the pathobiology of infectious microorganisms, as iron homeostasis and sequestration play an important role in host-pathogen interaction and defense mechanisms.

Abstract
Document type
Dataset
Creators
CreatorsAffiliationORCID
Danielli, AlbertoDept. of Pharmacy and Biotechnology - University of Bologna0000-0002-9414-9898
Keywords
Ferric Uptake Regulator, Helicobacter pylori, Nucleoid Associated Protein, chromatinn interactions
Subjects
DOI
Contributors
Name
Affiliation
ORCID
Type
Vannini, Andrea
Dept. of Pharmacy and Biotechnology - University of Bologna
Researcher
Rivetti, Claudio
Dept. of Chemistry, Life science and Environmental Sustainability - University of Parma
Researcher
Deposit date
17 Jul 2024 08:01
Last modified
17 Jul 2024 08:02
Project name
2010P3S8BR - Un approccio integrato per la comprensione dei determinanti strutturali e funzionali della patogenesi e persistenza di Helicobacter pylori
Funding program
Ministero dell'Università e della Ricerca - PRIN
URI

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