Abstract

This dataset contains data collected in the PoC MISE - AlmaValue project “LATONA” (“Lattobacilli della donna quali agenti bioterapeutici per promuovere il corretto sviluppo del microbiota intestinale e del sistema immunitario nel neonato”), funded by Ministero dello Sviluppo Economico on PNRR funds and within the framework of the European Commission's Next Generation EU programme. In particular, the research focused on the vaginal strain Limosilactobacillus vaginalis BC17 and its derivatives (cell-free supernatant and heat-killed cells), and some health-promoting features were considered: (i) L. vaginalis BC17 was indagated for the ability to adhere to intestinal cells (i.e. Caco-2 and HT-29 cells) and to survive in co-incubation with them. (ii) The absence of cytotoxicity of the strain and its derivatives was demonstrated on intestinal cells lines (i.e. Caco-2 and HT-29). (iii) Any genotoxicity effects of cell-free supernatant and heat-killed cells were excluded. (iv) L. vaginalis BC17 and its derivatives were sought for the capacity to favour the adhesion of beneficial Bifidobacterium spp. and to inhibit the adhesion of enteropathogens (i.e. enterotoxigenic E. coli, Salmonella enterica, and Yersinia enterocolitica) to Caco-2 cells. (v) Anti-inflammatory properties were highlighted both on intestinal cells and macrophages models. Moreover, the dataset provides the data relative to the development of freeze-dried tablets containing L. vaginalis BC17 or heat-killed cells in the presence of cell-free supernatant and maltodextrin. In particular, the dataset supplies raw data in terms of diameter, thickness and weight of tablets resulting from a screening procedure of different ratios of maltodextrin and cell-free supernatant. It also contains the characterization of the selected tablets in terms of diameter, thickness, weight, pH at dissolution, content uniformity, and disintegration time in different media at different temperatures. Moreover, the data set includes the stability of the selected tablets’ physical features (diameter, thickness, and weight), functional properties (impact on intestinal cells viability, impact on Bifidobacterium spp. and E. coli adhesion to Caco-2 cells, anti-inflammatory activities) and BC17 viability (only for the tablet containing the viable probiotic) over three months.