ECLIPSE. WP5 Task3. Comparing molecular methods for identification of Leishmania infantum in blood samples. V2

Gritti, Tommaso ; Varani, Stefania ; Mola, Beatrice ; Ortalli, Margherita ; Lazzarotto, Tiziana ; Nigrisoli, Giacomo ; Prodi, Luca (2025) ECLIPSE. WP5 Task3. Comparing molecular methods for identification of Leishmania infantum in blood samples. V2. University of Bologna. DOI 10.6092/unibo/amsacta/8767. [Dataset]
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Abstract

The following file contains data about clinical samples of peripheral blood collected from patient from the Metropolitan area of Bologna and standard samples of Leishmania infantum genomic DNA (reference strain JPCM5) tested by three different real-time PCR assays for the diagnosis of visceral leishmaniasis. Data contained in this dataset are both quantitative and qualitative and are contained in a database (file “ECLIPSE_WP5_T3_real-timePCR-LeishmaniaInfantum-BloodSamples_230925_V2.ods”) arranged into three single sheets: one “Clinical_Samples_Dataset” contains qualitative and quantititative data of the study obtained by the analysis of clinical samples, a second “Calibration_experiments_Dataset” that contains data about molecular diagnostic method calibration and raw analysis data of standard samples of Leishmania infantum genomic DNA and the third, “Dataset_column_description” that cointains explanation about the data contained in the other two sheets “Clinical_Samples_Dataset” and “Calibration_experiments_Dataset”. Specifically, this version of the dataset contains the updated version of the database. Moreover, a zip archive that contains in silico BLAST analysis about primers utilized for sample analysis was also added. The data were generated in the framework of the Horizon Europe project ECLIPSE.

Abstract
Tipologia del documento
Dataset
Autori
AutoreORCIDAffiliazioneROR
Gritti, Tommaso0000-0001-5473-6070Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Varani, Stefania0000-0003-0862-4937Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Mola, BeatriceDepartment of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Ortalli, Margherita0000-0001-5447-8817Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Lazzarotto, Tiziana0000-0003-3093-363XDepartment of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Nigrisoli, GiacomoDepartment of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Prodi, Luca0000-0002-1630-8291Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy01111rn36
Parole chiave
Leishmania infantum, Visceral leishmaniasis, kinetoplast minicircle DNA, real-time PCR, molecular diagnosis
Settori scientifico-disciplinari
DOI
Contributors
Contributor
ORCID
Tipo
Affiliazione
ROR
Argnani, Lisa
Researcher
Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy
Granozzi, Bianca
Researcher
Infectious Diseases Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
Bacchiega, Michele
Researcher
Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy
Melchionda, Fraia
Researcher
Pediatric Hematology and Oncology, IRCCS Azienda Ospedaliero-Universitaria di Bologna S. Orsola Hospital, Bologna, Italy
Dondi, Arianna
Researcher
Pediatric Emergency Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
Castaldini, Silvia
Researcher
Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy
Denwood, Matt
Researcher
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark
Varani, Stefania
Contact person
Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy
Data di deposito
02 Feb 2026 11:28
Ultima modifica
02 Feb 2026 11:29
Risorse collegate
Tipologia
Relazione
Identificativo
DOI
questo contributo è una nuova versione di
Nome del Progetto
ECLIPSE - ECL-based Infectious Pathogen (bio)SEnsor
Programma di finanziamento
EC - HE
URI

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