Experimental data on Analysis of ram sperm metabolism

Name: Experimental data on Analysis of ram sperm metabolism
Keywords: sperm metabolism, ram, seahorse, andrology

Algieri, Cristina ; Ramirez-Gonzalez, David ; Nesci, Salvatore ; Ortiz-Rodriguez, Jose Manuel ; Bucci, Diego (2026) Experimental data on Analysis of ram sperm metabolism. University of Bologna. DOI 10.6092/unibo/amsacta/8846. [Dataset]

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Abstract

Considering the differences on sperm metabolism among species, we aimed to use the innovative technology Seahorse XFp analyzer (Agilent), to assess both oxygen consumption rate (OCR), as an aerobic metabolism index, and extracellular acidification rate (ECAR), as a glycolytic index, in fresh ram spermatozoa, in order to delineate the main metabolic strategies used by ram sperm to obtain energy and relate these indexes with other sperm functional parameters such as mitochondrial membrane potential (assessed by JC-1) and sperm motility.

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Autori

Autore	ORCID	Affiliazione	ROR
Algieri, Cristina	0000-0002-1742-9378	University of Bologna	01111rn36
Ramirez-Gonzalez, David		Universidad de León	02tzt0b78
Nesci, Salvatore	0000-0001-8569-7158	University of Bologna	01111rn36
Ortiz-Rodriguez, Jose Manuel	0000-0002-3248-6493	University of Bologna	01111rn36
Bucci, Diego	0000-0001-6276-2674	University of Bologna	01111rn36