Nano-ImmunoEra. WP2 T2.1. ECL from beads on lasered SPCE

Santo, Claudio Ignazio (2024) Nano-ImmunoEra. WP2 T2.1. ECL from beads on lasered SPCE. University of Bologna. DOI 10.6092/unibo/amsacta/7883. [Dataset]
Full text disponibile come:
[thumbnail of Nano-ImmunoEra. WP2 T2.1. ECL from beads on lasered SPCE] Archivio (Nano-ImmunoEra. WP2 T2.1. ECL from beads on lasered SPCE)
Licenza: Creative Commons: Attribuzione 4.0(CC BY 4.0)

Download (91MB)

Abstract

This folder stores data related to Task 2.1 ECL from beads on lasered SPCE within Work Package 2 Production Characterization Materials for Improved ECL-based Biosensing, in the NanoimmunoEra Horizon 2020 project. The data includes ECL (Electrochemiluminescence) images and quantitative ECL results about 2.8 micrometer streptavidin-coated magnetic beads. We reported here an innovative approach to enhancing the ECL properties of screen-printed carbon electrodes (SPCEs) through post-fabrication CO2 laser treatment. By examining three different carbon paste-based electrodes with advanced ECL microscopy, we demonstrate that laser treatment at low energy doses (7–12 mJ/cm²) effectively improves the electrochemical properties of the material by removing surface binders and impurities. Quantitative analysis with a photomultiplier tube (PMT) revealed that GST electrodes can sensitively detect biomarkers with a limit of detection (LOD) of approximately 11 antibodies/µm². These findings underscore the potential of laser-treated GST electrodes for high-sensitivity ECL immunoassays and biosensing applications.

Abstract
Tipologia del documento
Dataset
Autori
AutoreAffiliazioneORCID
Santo, Claudio IgnazioUniversity of Bologna0000-0003-4802-1671
Parole chiave
Electrochemiluminescence, Disposable electrode, screen-printed carbon electrodes, biosensor
Settori scientifico-disciplinari
DOI
Contributors
Contributor
Affiliazione
ORCID
Tipo
Paolucci, Francesco
University of Bologna
Research group
Valenti, Giovanni
University of Bologna
Contact person
Data di deposito
17 Set 2024 07:52
Ultima modifica
18 Set 2024 13:52
Nome del Progetto
Nano-ImmunoEra - NANOTECHNOLOGY-ENABLED DETECTION OF CLINICALLY RELEVANT ANTIBODIES FOR EARLY CANCER DIAGNOSIS AND IMMUNOTHERAPY MONITORING
Programma di finanziamento
EC - HE
URI

Altri metadati

Statistica sui download

Statistica sui download

Gestione del documento: Visualizza il documento

^