Abstract

This dataset reports data obtained within the NanoimmunoEra project, Work Package 2 (Production and characterization of materials for improved ECL-based biosensing), specifically Task 2.4 (Electrochemical and ECL performance of ink-jet printed electrodes); Disposable ink-jet printed and screen-printed electrodes were tested to evaluate their influence on electrochemical and ECL performances, with optimization aimed at developing low-cost sensors. Early detection of cardiac troponin I (cTnI) demands ultrasensitive electrochemiluminescence (ECL) platforms to enable timely intervention and better prognosis. Here, screen-printed carbon electrodes (SPCEs) were modified by drop-casting Cu₃(HHTP)₂ conductive metal-organic frameworks, yielding a highly responsive sensing substrate that was further engineered with anti-cTnI antibodies and BSA passivation for specific target capture.​ Using [Ru(bpy)₃]²⁺ as luminophore, the Cu₃(HHTP)₂-modified interface generated markedly intensified ECL signals versus bare SPCEs, driven by the material's superior electrical conductivity, extensive porosity, expanded electroactive area, and—most importantly—the ECL-promoting action of HHTP ligands.​ Performance calibration against graded cTnI standards revealed exceptional analytical figures of merit, including an in vitro limit of detection of 10.23 ± 1.06 pg mL⁻¹—far surpassing clinical decision limits. This co-reactant-independent Cu₃(HHTP)₂ ECL immunosensor thus emerges as a promising, high-efficiency tool for swift point-of-care assessment of low-abundance cardiac markers.​