Abstract

This dataset contains the raw data collected from an aquarium experiment investigating the combined effects of temperature, pH, and salinity on the Mediterranean bivalve Chamelea gallina. The Excel file is organized into multiple sheets, each corresponding to a specific type of analysis, and includes measurements of mortality, condition index, skeletal properties, mechanical properties, and microstructural characteristics of the shells. Recorded values of temperature, pH, and salinity during the experimental treatments are also included. One sheet also reports carbonate chemistry data derived from the aquarium measurements of pH, salinity, temperature, and total alkalinity, calculated using the CO2SYS software. The dataset supports statistical and modeling analyses and enables further exploration of the physiological, mechanical, and mineralogical responses of bivalves to controlled environmental stressors. The data underpin the study reported in Mancuso & Bardone et al. (2026), Triple threat: Ocean acidification, warming, and hyposalinity synergistically weaken shell integrity in a Mediterranean calcifying mollusk, published in Marine Pollution Bulletin.