Abstract

This dataset contains data describing the isolation, expansion, and characterization of mesenchymal stromal cells (MSCs) derived from fresh and cryopreserved equine Wharton's jelly and canine umbilical cord tissues. Data were generated to evaluate the feasibility of whole-tissue cryopreservation as a biobanking strategy and to assess its impact on MSC recovery and biological properties. MSCs were isolated from fresh tissues or from tissues cryopreserved using different cryoprotective solutions and subsequently expanded in vitro. The dataset includes cell yield after primary isolation, growth kinetics assessed by population doubling time (PDT), clonogenic potential evaluated by colony-forming unit (CFU) assay, spheroid formation parameters (area and volume), and migration capacity determined by wound-healing assays. In addition, the dataset contains quantitative data from adipogenic, osteogenic, and chondrogenic differentiation assays, expressed as normalized differentiation ratios. These data were used to compare the biological and functional properties of MSCs obtained from fresh and cryopreserved tissues in both equine and canine species.