Abstract

In the human spleen, encapsulated bacteria are first captured by CD206+ sinusoidal lining cells and subse-quently transferred to CD163+ red pulp macrophages for killing, revealing a previously unrecognized two-step immune defence. We deposited the data supporting this study, including high-resolution imaging, 3D reconstructions, and time-lapse microscopy of human spleen sections and primary splenic cell cultures, showing the spatial organization and dynamic interactions of macrophages with sinusoidal lining cells for bacteria uptake and elimination. Specifically, the dataset includes high-content slide-scanning microscopy, confocal immunofluorescence, 3D reconstructions, and time-lapse macrophage tracking in the presence or absence of mannose during infection.