Abstract

Ovarian cancer (OC) poses significant treatment challenges due to late-stage diagnosis and a complex tumor microenvironment contributing to therapy resistance. We here deposited the data which allowed optimization of a U-CUP perfusion-based bioreactor method to culture patient-derived primary and metastatic OC specimens, demonstrating that perfusion better preserves cancer cell viability and proliferation, both when fresh and slow-frozen tissues were used. Specifically, histology analyses include: hematoxylin/eosin staining, PAX8 immunohistochemistry, Ki67/ECad immunofluorescence, αSMA/CD31 immunofluorescence, CD45 immunohistochemistry, D2-40 immunohistochemistry