Data relative to histology analyses from the publication "Perfusion-based ex vivo culture of frozen ovarian cancer tissues with preserved tumor microenvironment”

De Luise, Monica (2025) Data relative to histology analyses from the publication "Perfusion-based ex vivo culture of frozen ovarian cancer tissues with preserved tumor microenvironment”. University of Bologna. DOI 10.6092/unibo/amsacta/8274. [Dataset]
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Abstract

Ovarian cancer (OC) poses significant treatment challenges due to late-stage diagnosis and a complex tumor microenvironment contributing to therapy resistance. We here deposited the data which allowed optimization of a U-CUP perfusion-based bioreactor method to culture patient-derived primary and metastatic OC specimens, demonstrating that perfusion better preserves cancer cell viability and proliferation, both when fresh and slow-frozen tissues were used. Specifically, histology analyses include: hematoxylin/eosin staining, PAX8 immunohistochemistry, Ki67/ECad immunofluorescence, αSMA/CD31 immunofluorescence, CD45 immunohistochemistry, D2-40 immunohistochemistry

Abstract
Document type
Dataset
Creators
CreatorsAffiliationORCID
De Luise, MonicaDepartment of Medical and Surgical Sciences, University of Bologna0000-0002-3486-3985
Keywords
Ovarian Cancer, Perfusion Bioreactor, Tumor Microenvironment, TME, Ex Vivo Culture, 3D culture, Chemotherapy Response, QuPath
Subjects
Area 05 - Scienze biologiche > BIO/17 Istologia
Area 06 - Scienze mediche > MED/04 Patologia generale
Area 06 - Scienze mediche > MED/06 Oncologia medica
Area 06 - Scienze mediche > MED/40 Ginecologia e ostetricia
DOI
10.6092/unibo/amsacta/8274
Contributors
Name
Affiliation
ORCID
Type
Kurelac, Ivana
Department of Medical and Surgical Sciences, University of Bologna
Researcher
Gasparre, Giuseppe
Department of Medical and Surgical Sciences, University of Bologna
Contact person
Muraro, Manuele Giuseppe
University of Basel and University Hospital of Basel, Dept. of Biomedicine, Tissue Engineering
Contact person
Deposit date
12 Jun 2025 08:52
Last modified
12 Jun 2025 10:15
Related identifier
Related identifier type
Relation type
Code
DOI
this upload is supplement to
Project name
AMICO - Assessing the Efficacy of Mitochondrial Complex I Targeting in Combined Therapies for Ovarian Cancer
Funding program
Associazione Italiana Ricerca sul Cancro (AIRC) - Investigator Grant (IG)
URI
https://amsacta.unibo.it/id/eprint/8274

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© ALMA MATER STUDIORUM - Università di Bologna, 2007-2025.

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